不同产区麦冬、山麦冬rDNA-ITS序列分析

rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions

摘要: 利用特异性引物进行PCR扩增和克隆、测定不同产区麦冬和山麦冬原植物的18S～26S r DNA-ITS区碱基序列,结果得到供试的7个不同产区麦冬和山麦冬植株样品rDNA的ITS及5.8SrDNA全序列、18S和26SrDNA部分序列,序列长度在696～711 bp。通过分析比较各ITS序列,表明不同产区麦冬和山麦冬rDNA-ITS区存在差异,可为其分子鉴别提供了依据。

Abstract: The 18S-26S rDNA-ITS base sequences of Radix ophiopogonis and Radix liriopes from different geographical regions were compared by using the specific PCR-ITS.The complete sequences of ITS and 5.8S rDNA,as well as partial sequences of 18S rDNA and 26S rDNA,in the range of 696-711 bp for the seven samples were obtained.By comparing the sequences,it was apparent that the ITS sequences of R. ophiopogonis and R. liriopes from different regions varied.Our analytical results provided a basis for molecular identification of the species.