• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

油樟中呋喃型木脂素对层出镰刀菌的抑制活性及作用机理研究

Inhibitory Activity of Cinnamomum longepaniculatum Lignans on Growth of Fusarium proliferatum

    摘要
  • 摘要:
    目的 探讨油樟呋喃型木脂素对植物病原真菌层出镰刀菌的抑菌活性，为植物源农药先导物的发现及油樟资源开发利用奠定基础。
    方法 采用孢子萌发和菌丝生长速率法测定油樟呋喃型木脂素对层出镰刀菌的抑制活性，初步阐明构效关系；深入研究优选出的活性分子对层出镰刀菌的形态、干重、细胞膜电导率、菌体蛋白质、丙二醛和还原糖含量及多种保护酶系活性的影响，初步探讨呋喃型木脂素可能的作用机制。
    结果 呋喃型木脂素对层出镰刀菌有较好的抑制作用，在药剂浓度为100 µg·mL−1时，其中四氢呋喃型木脂素对层出镰孢菌菌丝生长的抑制率在16.7%~75.2%，；活性分子(+)-piperitol 的EC50值为38.615 µg·mL−1；经 (+)-piperitol处理后，层出镰刀菌菌丝形态受到影响，干重显著降低，细胞膜电导率显著增加，菌体蛋白质含量显著降低，丙二醛含量显著增加，还原糖含量显著降低，多种保护酶系活性显著降低。
    结论 油樟呋喃型木脂素具有良好的抑菌活性，与化学结构显著相关，可抑制菌丝生长和孢子萌发，机制与影响菌丝细胞膜正常功能、降低保护酶系活性等有关。

     

    Abstract:
    Objective Antifungal activity of furan-type lignans from Cinnamomum longepaniculatum on Fusarium proliferatum was investigated for utilization of the natural resource.
    Methods Inhibitory effects of C. longepaniculatum lignans on spore germination and mycelial growth of F. proliferatum was tested to determine the structure-activity relationship (SAR) between the molecule and its biological activity. Subsequently, effects of the active chemical components on the mycelial morphology, dry weight, cell membrane conductivity, protein content, malondialdehyde (MDA), and reducing sugars as well as protective enzyme activities of the fungus were analyzed to understand the inhibition mechanism.
    Results The tetrahydrofuran-type lignans of C. longepaniculatum exhibited potent antifungal activity on F. proliferatum. At the dosage of 100 µg·mL−1, it inhibited 16.7-75.2% mycelial growth of the fungus. The active (+)-piperitol in the lignans showed an EC50 of 38.615 µg·mL−1. Under the treatment, significantly, the fungal mycelia was morphologically altered, dry weight decreased, cell membrane conductivity raised, intracellular protein content declined, MDA increased, reducing sugar lowered, and activities of multiple protective enzyme systems reduced.
    Conclusion The furan-type lignans from C. longepaniculatum displayed a potent in vitro antifungal activity on F. proliferatum. The efficacy appeared to closely correlate to its chemical structure, as indicated by the SAR analysis. The functions of mycelial cell membranes of the fungus were disrupted and the protective enzyme activities downregulating by the (+)-piperitol treatments resulting in inhibition of spore germination and retardation of mycelial development of the pathogen.

     

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