水禽源禽1型副黏病毒强毒RT-PCR方法的建立

基金项目:

国家自然科学基金项目(30671564);国家“973”重大基础研究专项子课题(2005CB523001);福建省科技重大专项子课题(2006NZ0003-2)

详细信息

Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China;
3.
Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China

摘要: 参照GenBank上登录的水禽源禽1型副黏病毒强毒株F基因序列设计引物,建立检测水禽源禽1型副黏病毒强毒株的RT-PCR方法。该方法能从水禽源禽1型副黏病毒强毒株扩增出1条400 bp的特异片段,从水禽源禽1型副黏病毒弱毒、鸭瘟病毒、鸭1型肝炎病毒、减蛋综合征病毒、传染性法氏囊病病毒、H9亚型禽流感病毒和禽多杀性巴氏杆菌等均不能扩增出目的片段。敏感性试验显示该RT-PCR方法最低可检测出10 pg的病毒核酸。因此,该RT-PCR方法可用于水禽源禽1型副黏病毒强毒的临床诊断。
- 水禽源禽1型副黏病毒 /
- 强毒株 /
- RT-PCR
Abstract: An RT-PCR method was developed to detect virulent strains of waterfowl-origin avian paramyxovirus type 1(vwAPMV-1).The primers were designed according to the sequences of the fusion protein gene of vwAPMV-1s available at the GenBank.The result showed that the 400 bp of specific fragments were amplified in vwAPMV-1.However,negative results were obtained from Pasteurella multocide,EDS,DEV,IBDV,H9N2 influenza virus,DHV-1 and low virulent strains of waterfowl-origin avian paramyxovirus type 1.And,the 10pg of vwAPMV-1 RNA was amplified by the sensitivity test for the RT-PCR.The results indicated that the RT-PCR could be used for vwAPMV-1 detection.
- waterfowl-origin avian paramyxovirus type 1 /
- virulent strains /
- RT-PCR

1.	闫世雄，张芯燕，简宗辉，李颜攸，柏毅承，胡梅，豆腾飞，贾俊静. 鸡脂肪代谢的分子调控. 中国家禽. 2022(04): 90-100 . 百度学术
2.	吴正明，龚绍荣，赵加鼎，邱丙姗，李再磊，程娜，李文君，周戚斌，赵桂英. FASN基因在保山猪3个组织中的表达量与背肌营养物质含量的关联分析. 中国畜牧杂志. 2022(08): 229-234 . 百度学术
3.	樊庆灿. 鸡肝脏表达谱数据分析筛选脂肪沉积相关基因. 中国畜牧杂志. 2020(10): 85-88+94 . 百度学术
4.	梁金辉，杨娜娜，李梦柔，唐可人，郭新颖，祁雨田，强巴央宗，商鹏. FASN基因在不同品种猪背脂、肝脏、背最长肌组织中表达模式研究. 高原农业. 2019(01): 65-69+83 . 百度学术