Cloning and functions of a zinc-finger gene promoter preferentially expressed in rice stems and leaves
-
摘要: 通过RT-PCR分析,鉴定了一个在水稻茎、叶中特异表达的锌指基因OsZF153。利用PCR扩增了OsZF153基因上游约2 kb启动子区,构建了启动子与GUS的融合表达载体pCZF153P-GUS。pCZF153P-GUS转基因T0植株GUS活性分析显示:转基因植株的根、茎、叶、颖壳中检测到GUS活性,其中茎和叶GUS表达活性较强,而根和颖壳表达较弱,在雄蕊和雌蕊及种子不同发育阶段中没有检测到GUS活性,表明OsZF153启动子是一个水稻非生殖器官表达,且在茎、叶中优势表达的启动子。Abstract: A zinc-finger gene(tentatively named OsZF153),which was preferentially expressed in the rice stems and leaves,was identified by RT-PCR analysis.A fusion vector was constructed by combining approximately 2 kb upstream from the start codon of the gene OsZF153 with the reporter gene GUS.The GUS activity was detected in the T0 generation of transgenic rice plants.A strong GUS activity could be detected in the stems and leaves,while a weak one in the roots and hull,of the genetically modified rice.But no GUS activity was observed in stamen,pistil,embryo or endosperm of the transgenic rice.The results suggested that OsZF153 promoter was a vegetative organ promoter,which was preferentially active in stems and leaves of rice.
-
Keywords:
- rice /
- zinc-finger gene /
- OsZF153 /
- stem and leaf /
- preferential expression
-
-
RIL G,LI Z,GUO Y W. Isolation of a maize betaglucosidase gene promoter and characterization of its activity in transgenic tobacco [J]. Plant Cell,2006,25(11): 1157- 1165.
MARRACCINI P,COURJAULT C,CAILLET V,et al. Rubisco small subunit of Coflea arablca: cDNA sequence,gene cloning and promoter analysis in transgenic tobacco plants [J]. Plant Physiology and Biochemistry,2003,41: 17- 25.
计量
- 文章访问数: 2293
- HTML全文浏览量: 105
- PDF下载量: 1254
下载:
