Abstract: Effects of hormones and activated carbon in medium on the proliferation and rooting of Phyllagathis fordii were studied for the culture optimization.Plant tissues from aseptic seedlings were used as the explants for the orthogonal design experiment L9(34).The result showed that (a) MS supplemented with 6-BA 1.2 mg·L-1＋NAA 0.4 mg·L-1＋KT 0.1 mg·L-1 was appropriate for the proliferation; (b) 1/2 MS supplemented with IBA 0.5 mg·L-1+activated carbon 1.0 g·L-1 was suitable for rooting; and (c) the survival rate were greater than 90% when the plantlets were transplanted to a soil medium containing 2/3 peat and 1/3 perlite.