摘要:
为建立一种欧洲鳗鲡病毒性疾病分离、鉴定的细胞模型,深入了解鳗鲡病毒性疾病流行与传播的情况,本试验在欧洲鳗鲡胸鳍传代细胞体外培养的最适条件下,通过添加碱性成纤维细胞生长因子(bFGF)和胰岛素样生长因子1(IGF-1)等可能的传代细胞促长因子,并在限定的时间内收集细胞、进行细胞计数、绘制胸鳍细胞的生长曲线,分析其对欧洲鳗鲡胸鳍细胞生长速率的影响。试验结果提示:在10%FBS L-15全价培养基的条件下,bFGF对欧洲胸鳍细胞的生长有明显的促进作用,最佳效应浓度为10 μ·L-1;IGF-1(5~50 μg·L-1)不利于欧洲鳗鲡胸鳍细胞的生长。
Abstract:
To establish a model for cell isolation and identification, as well as to gain an in-depth understanding of the viral diseases in Anguilla anguilla, the roles played by the growth factors, bFGF and IFG-1, on the proliferation of the eel's pectoral fin cells were studied.Under in vitro conditions, the cells were collected, counted and growth curves constructed.On the L-15 medium containing 10% FBS, bFGF showed a significant evidence in promoting the growth of the pectoral fin cells.The cellular growth peaked at bFGF concentration of 10 μg·L-1.On the other hand, IGF-1 in the concentrations from 5 μg·L-1 to 50 μg·L-1 did not show any apparent effect on the growth.Therefore, a culture medium formulation for effective proliferation of the A.anguilla pectoral fin cells was obtained.This would, in turn, aid future studies on the pathogen's isolation, identification and vaccine preparation.
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