秦始皇兵马俑1号坑芽胞杆菌的采集与鉴定
Identification and Phylogenetic Analysis of Bacillus Isolated from Soil at 1st Pit Site of Emperor Qin's Terracotta Warriors
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摘要: 芽胞杆菌分布广泛,世界各地到处都有芽胞杆菌的踪迹。【目的】了解秦始皇兵马俑坑中土芽胞杆菌的多样性,丰富特殊环境下芽胞杆菌的地理分布。【方法】从秦始皇兵马俑1号坑中采集土壤样品,采用温度筛选法和稀释梯度涂布法分离到了10株芽胞杆菌,通过16S rRNA作为鉴定手段对这10株芽胞杆菌进行鉴定。【结果】通过16S rRNA 将10株芽胞杆菌鉴定为4种芽胞杆菌,巨大芽胞杆菌1株、地衣芽胞杆菌1株、梭状芽胞杆菌1株、枯草芽胞杆菌5株,另外2株为芽胞杆菌疑似新种。【结论】研究结果表明秦始皇兵马俑1号坑土中存在着丰富的芽胞杆菌种类,丰富了我国极端环境下芽胞杆菌资源地理分布。Abstract: Bacillus bacteria are widely distributed around the world.This study examined the diversity of Bacillus in the pit soil at Terracotta Warriors site and the geographical distribution of the bacteria in the specific environment.Soil samples were collected from the 1st pit of the Terracotta Warriors and subjected to a temperature screening and dilution plating to obtain 10 strains of Bacillus.These strains were subsequently identified by their 16S rRNAs to belong to 4 species and two possibly new Bacillus species.The 16S rRNA similarity among the strains was more than 99%.Thus, abundant Bacillus species were found to exist in the soil of the Terracotta Warriors site, and that the geographic distribution enriched the national microbial resource.
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