Abstract: For establish a cell model of Anguilla Anguilla which can be used for isolation and identification of Anguilla Anguilla virus, the pectoral fin cells of Anguilla Anguilla (the 21st generation) were used in this study. The pectoral fin cells of Anguilla Anguilla were cultured in artificial medium in vitro to explore the optimum conditions. The pectoral fin cells of Anguilla Anguilla were cultured under different mediums (L-15, RPMI-1640, and DMEM), different serums (BS, FBS), and different serum concentrations (5%, 10%, 15%, and 20%). In different time points, the cells were collected and counted, and then the growth curves of the cells were drawn. The effects of the different conditions on the proliferation of the pectoral fin cells were evaluated by the growth curves. The results showed the L-15 was the optimum medium,while RPMI-1640 took the second place and DMEM was the poorest; FBS was better than BS, while 10%FBS was the best concentration.