Abstract: H3N2 swine influenza virus inactivated vaccines were prepared with IMS1315 adjuvant and oil emulsion adjuvant. Sows and piglets, which were antibody negative of H3N2 swine influenza virus, were selected and vaccinated two times at intervals of 21days with both inactivated vaccines. Antibodies were determined 12 times at the immune period of 171days with HI. The results showed that both in sows and piglets, the HI positive antibodies were detected at the 7th day after vaccination with IMS1315 adjuvant vaccine and were detected at the 14th day after vaccination with oil emulsion vaccine. The antibody peak levels were detected at the 28th day after second vaccination of both inactivated vaccines. They were 11.0 Log2, 10.2 Log2 in sows and 10.2 Log2, 10.0 Log2 in piglets, respectively. The antibody levels could be maintained in 6.5Log2 and 6.0Log2 at the 150th day after the second vaccination, respectively. It indicated that the effect of IMS1315 adjuvant inactivated vaccine was better than that of oil emulsion inactivated vaccine.