Abstract: The designed method of split-split-plot has been applied to study the effect of genotype, row spacing and the number of plants per hill on the yield in spring soybean the results show that pudon 8008 is a good variety with high-yielding characteristics and it is feasible to be popularized in spring soybean area in Fujian. Furthermore, it is essential to make high yield by row spacing 0.8M and 3 plants per hill in spring soybean. It can fully utilize border effect, and also maintain pipulation with high yield about 25?103 plants per mu in spring soybean.