Abstract: To counter this tendency with low yield and high polluted rate of Jew’s-ear by sugarcane bagasse, we carried out appraisal test of 7 Jew’s-ear strains. It is determinated that the strain D-5 passesses high yielding and fine quality. The vigour of its hypha is strong. The hypha grows in 4mm. on an average in day and 0.7-1.2mm. longer than other 6 strains. The fruit-body of the Jew’s-ear leaf of D-5 is large. Its flesh is thick and tincture is black. On an everage the substance of 100kg. produces dry Jew’s-ear over 6 kg. Its yield increased in 50% than entered test strain D-8.which is popularized by culture of substitute materials in our country.And its yield increased in 88.9% than entered test strain D-7, which is popularized by culture of log in our country.The fillprescription of 0.5% urea instead of 50% wheat bran has obvious effecient for increasing yield. The bio-efficiency reached by 74%. It increased 21.1% yield than control-prescription with adding 20% of wheat bran. And pollute rate reduced from 34% to 10%-indicating that urea is a fine nitrogen resource for growth of Jew’s-ear. Its rate of best combination is 0.5% urea required amount and 10% required amount of wheat bran. It is save on food and cost.