Abstract: Sixteen strains of Colletotrichum indicum were isolated from the infected kenaf samples collected from 4 provinces in China.Sporulation and pathogenicity of 5 representative strains of them were tested.The results showed that the m-XS1 strain was the most pathogenic species and produced the greatest number of spores.Its infection occurred mainly on the wounded kenaf and matured leaves.The strain was inoculated on 11 kenaf varieties,with or without wound,in a greenhouse.All tested varieties were infected by C.indicum under high humidity,with 3 of them were nearly immune to the disease.It was significantly correlated to kenaf’s disease indices,with or without wound(r=0.774).The same treatments on 160 kenaf varieties were performed in the field.Seven kenaf varieties were highly resistant to the disease.They could be used as high resistant kenaf parents for further research.