摘要:
本研究以GeO2为锗源,通过仿野生栽培得到富锗灵芝于实体,生物学效率为8.63%～9.68%.经石墨炉原子吸收法测定,当年生子实体含锗量为0.22～8.25μg/g,对锗元素的吸收利用率为0.02%～0.19%;而次年生子实体含锗量为6.60～138μg/g,吸收利用率达1.68%～6.73%.氨基酸分析结果表明,富锗灵芝全氨基酸含量为7.18～8.32mg/100mg,比对照的普通灵芝高19.0%～37.8%;富锗灵芝的必需氨基酸含量较丰富,E/T=0.577～0.625;其中,蛋氨酸(Met)含量提高一个数量级,高达2.09～2.64mg/100mg.据此推测,灵芝于实体在代谢水平上产生与富锗相关的生理变化,显着影响到氨基酸尤其是蛋氨酸的合成代谢。
关键词:
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灵芝
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子实体
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富锗
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吸收利用率
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氨基酸
Abstract:
Germanium dioxide was applied as additive to obtain Ge-enriched fruitbodies of Ganoderma lucidum by Imitate -wild-culture method and its biological efficiency were 8.63%~9.68%.Gecontents in the fruitbodies harvested in same year were 0.22~8.25 μg/g analysed by FAAS, and Geuptaked rates were 0.02 % ~0.16%;the fruitbodies harvested in the second year,Gecontentswere 6.60~138μg/g,the rates were 1.68%~6.73%.Amino acid analysis showed that, the content of total amino acids in Geenricned iruitbodies were 7.18~8.32 mg/100 mg(dry matterr)which were 19.0%~37.8%higher than normal fruitbcxlies;the ratio of essential aminoacid of Geenriched fruitbodies were higher, E/T=0.577~0.625;among that,methionine (Met)contentswere 2.09 mg~2.64 mg/100 mg (dry matter),raised about one quantitative degree.Accordingly,we speculated that there was a certain kind of physiological change related to the metabolism levelof Geenrichment in the fruitbodies,especially a notable effect on synthetic metabolism ofaminoacids.
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