Abstract: Seventeen strains of Grifola frondosa were collected from. China and foreign countries. Indoor and outdoor experiments showed that,GF17 belonged to middle temperatural type,its fruitbodies formed and developed at 17～26℃,the optimum temperature was 21～22℃;early maturity,white colour,the average fresh weight of single fruitbody was 143.5g,bearing rate reached 95% or more;GF5 was mid-low temperatural type,grew and developed at 16～20℃,optimum was 18℃,middle maturity,black colour,fresh single fruitbody weighted 116.5 g,bearing rate was more than 90%.GF11 and. GF6 were low temperatural type,grew at 14～19℃,the optimum was 16℃,late maturity,grey colour,average fruitbody weights were 151.5 and 119.5 respectively,bearing rate reached 85% or more.The analysis results showed that various. GF strains differ in their amino acid contents.The illumination intensity was an important factor for the colour-forming and quality of fruitbodies.