Chemical Differentiations of Spring, Summer, and Autumn 'Jinmudan' Teas
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摘要:目的
揭示‘‘‘金牡丹’茶的主要品质特征及其春、夏、秋3季中不同茶类（红茶、乌龙茶、白茶、黄茶及绿茶）的主要品质成分差异情况，探究不同茶季金牡丹茶主要特征代谢物间的关联特性以及可用于区分不同茶季的主要代谢物。
方法以不同茶季的金牡丹鲜叶制成的红茶、乌龙茶、黄茶、白茶及绿茶为供试材料，基于生化测定、高效液相色谱法以及AQC（6-aminoquinolyl-N-hydroxysuccinimidyl carbamate）衍生液质联用法对供试茶样的生化成分进行测定分析。
结果金牡丹夏茶的茶多酚和儿茶素含量高于其他茶季。而春茶中氨基酸含量最高，夏茶最低。金牡丹茶的儿茶素组成以EGC、EGCG、ECG及GC为主。相关性分析表明，不同茶季中特征性代谢物间显著相关。基于OPLS-DA判别模型，在不同茶季的金牡丹红茶、乌龙茶、白茶、黄茶及绿茶中分别筛选出12、15、15、17、17种差异代谢产物，这些代谢物可作为区分春、夏、秋茶的潜在标志。
结论本研究明确了金牡丹茶在不同茶类和茶季中的滋味品质化学特征，揭示其不同茶季的主要特征代谢物的变化规律，为提升金牡丹鲜叶原料的利用率及优化金牡丹茶品质提供科学依据。
Abstract:ObjectiveDifferentiations in the main chemicals related to quality of spring, summer, and autumn Jinmudan teas were analyzed to examine the correlations between metabolites and characteristics of the teas.
MethodsBlack, oolong, yellow, white, and green Jinmudan teas made of fresh leaves plucked separately in three seasons were analyzed for biochemical composition using the conventional methods, high performance liquid chromatography, and AQC derivative liquid chromatography tandem triple quadrupole mass spectrometry.
ResultsThe contents of tea polyphenols and catechins in the Jinmudan summer tea were significantly higher than those in the others, whereas that of amino acids highest in the spring tea and lowest in the summer tea. The catechins in the Jinmudan teas were mainly EGC, EGCG, ECG, and GC. Significant correlation between characteristic metabolites in different tea seasons. As analyzed by the OPLS-DA discriminant model, 12 differential metabolites came from the black tea, 15 from the oolong tea, 15 from the white tea, 17 from the yellow tea, and 17 from the green tea. These metabolites were distinctive enough to serve as markers to separate these teas.
ConclusionThe chemical differences among the varieties of spring, summer, and autumn Jinmudan teas underscored a close relationship between the chemistry and quality characteristics of the teas.
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Keywords:
- Jinmudan tea /
- types of tea /
- tea plucking season /
- sensory quality /
- chemistry
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图 1 不同茶季金牡丹茶主要特征代谢物相关性分析
春茶（A）、夏茶（B）、秋茶（C）。*代表0.01＜P≤0.05，**代表 0.001＜P≤0.01，***代表0.0001＜P≤0.001。
Figure 1. Correlation analysis of major characteristic metabolites in Jinmudan tea in different tea seasons
Spring tea (A), Summer tea(B), and Autumn tea (C). * indicates statistical significance at 0.01＜P≤0.05; ** at 0.001＜P≤0.01; and *** at 0.0001＜P≤0.001.
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图 2 不同茶季金牡丹5大类茶主要特征代谢物OPLS-DA与聚类热图综合分析
1~3分别为OPLS-DA得分图、OPLS-DA置换检验图及VIP聚类热图；A：红茶，B：乌龙茶，C：白茶，D：黄茶，E：绿茶。SP表示春茶；SU表示夏茶；AU表示秋茶；BT表示红茶，OT表示乌龙茶，WT表示白茶，YT表示黄茶，GT表示绿茶。
Figure 2. Comprehensive analysis of OPLS-DA and cluster heat map of main characteristic metabolites of multiple tea types of Jinmudan in different tea seasons
1–3: OPLS-DA score plot, OPLS-DA permutation test plot, and VIP cluster heatmap; A: black tea, B: oolong tea, C: white tea, D: yellow tea, E: green tea. SP represents spring tea, SU represents summer tea, AU represents autumn tea, BT represents black tea, OT represents oolong tea, WT represents white tea, YT represents yellow tea, and GT represents green tea.
表 1 不同茶季金牡丹5大茶类主要生化成分含量
Table 1 Biochemical compositions of 5 types of Jinmudan tea of different seasons
生化成分
Biochemical composition茶季
Tea season红茶
Black tea乌龙茶
Oolong tea白茶
White tea黄茶
Yellow tea绿茶
Green tea水浸出物
Water extract/%春茶 47.23±0.58c 46.31±0.26c 52.00±0.61b 54.01±0.34a 53.14±0.83a 夏茶 43.56±0.22d 46.73±0.13c 51.05±0.72b 53.10±0.83a 53.97±0.50a 秋茶 39.40±0.01d 45.41±0.01c 49.47±0.01b 51.95±0.00a 53.32±0.00a 茶多酚
Tea polyphenols/%春茶 18.14±0.64e 23.66±0.40d 26.60±0.12c 28.92±0.62b 31.34±0.32a 夏茶 20.00±0.61d 25.40±0.43c 31.03±0.40b 31.87±0.28b 34.63±0.16a 秋茶 17.44±0.51e 25.22±0.67d 29.31±0.42c 30.86±0.31b 33.02±0.41a 氨基酸总量
Total amount of amino acids/%春茶 1.72±0.06e 2.69±0.12d 4.64±0.07a 3.01±0.07c 3.95±0.16b 夏茶 1.24±0.03e 1.72±0.04d 2.90±0.06b 1.87±0.01c 3.14±0.05a 秋茶 1.60±0.03d 2.35±0.08c 4.04±0.20a 2.42±0.03c 3.56±0.08b 酚氨比
Phenol ammonia ratio春茶 10.53±0.61a 8.80±0.28b 6.24±0.09d 8.83±0.24b 8.23±0.43c 夏茶 16.14±0.84a 14.80±0.64c 10.66±0.24d 15.70±0.24b 10.62±0.04d 秋茶 10.88±0.30b 10.74±0.46c 7.27±0.36e 12.74±0.41a 9.28±0.35d 茶黄素
Theaflavin/%春茶 0.21±0.01a 0.04±0.01b 0.04±0.00b 0.03±0.00c — 夏茶 0.14±0.01a 0.03±0.00b 0.02±0.00c 0.02±0.00c 秋茶 0.10±0.00a 0.04±0.00b 0.03±0.00c 0.03±0.00c 茶红素
Thearubigin/%春茶 2.26±0.07a 1.61±0.09b 1.20±0.02d 1.23±0.07c — 夏茶 1.87±0.07a 1.38±0.10b 0.77±0.03d 1.08±0.08c 秋茶 2.36±0.16a 1.44±0.00b 0.93±0.02d 1.15±0.03c 茶褐素
Theabrownin/%春茶 1.92±0.04a 1.17±0.04b 0.77±0.04d 1.12±0.02c — 夏茶 2.10±0.03a 1.13±0.04b 0.75±0.01d 0.92±0.05c 秋茶 1.63±0.00a 1.62±0.01b 0.90±0.02d 1.05±0.08c “—”表示未检测出；相同小写字母表示同一茶季不同茶类之间数据差异不显著（P＞0.05），不同小写字母表示差异显著（P＜0.05），下同。
“—” indicates substance not detected; the same lowercase letters indicate no significant difference among different tea types of the same season (P＞0.05), while different lowercase letters indicate significant differences (P＜0.05). same for the following tables.
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表 2 不同茶季金牡丹5大类茶儿茶素及生物碱含量
Table 2 Catechin and alkaloid contents in 5 types of Jinmudan tea of different seasons
项目
Item组分名称
Component茶季
Tea season红茶
Black tea乌龙茶
Oolong tea白茶
White tea黄茶
Yellow tea绿茶
Green tea儿茶素
Catechin/（mg·g−1）EGC 春茶 1.53±0.16d 11.59±0.38c 9.26±0.28bc 18.03±0.78a 13.05±0.89b 夏茶 1.32±0.08e 19.18±1.76c 7.66±0.52d 23.60±1.75b 30.92±1.70a 秋茶 1.46±0.07d 16.85±0.31b 7.78±0.13c 17.21±0.55b 19.76±0.47a C 春茶 1.24±0.04d 1.37±0.04c 1.93±0.13b 1.84±0.01b 2.53±0.10a 夏茶 1.47±0.06e 1.62±0.06d 2.48±0.05b 2.29±0.06c 4.68±0.11a 秋茶 1.51±0.07c 1.61±0.04c 2.05±0.15ab 1.88±0.01bc 2.30±0.04a EGCG 春茶 1.82±0.19d 27.83±1.15c 60.73±0.92b 56.03±0.78b 72.25±0.74a 夏茶 3.26±0.06d 40.87±0.85c 77.61±0.70b 63.21±1.92b 90.72±0.19a 秋茶 1.04±0.17e 28.48±2.20d 58.80±1.17b 50.42±3.14c 67.27±2.81a EC 春茶 0.58±0.13d 5.16±0.38b 2.22±0.16c 6.03±0.52a 6.56±1.17a 夏茶 0.22±0.01e 6.69±0.45c 1.95±0.31d 8.19±0.48b 10.75±0.08a 秋茶 0.15±0.03c 5.28±0.35a 2.48±0.1b 5.74±0.54a 5.92±0.59a 儿茶素
Catechin/（mg·g−1）EGCG3″Me 春茶 0.10±0.01d 4.16±0.38c 5.36±0.21b 4.36±0.37c 6.20±0.05a 夏茶 0.69±0.07c 4.17±0.42b 3.97±0.69b 5.94±0.53a 6.35±1.14a 秋茶 0.64±0.17c 5.21±0.70a 5.34±0.25a 3.72±0.33b 4.05±0.42b ECG 春茶 1.61±0.06e 6.50±0.36d 20.70±0.10a 14.35±0.71c 15.84±0.81b 夏茶 2.81±0.09e 10.74±0.26d 16.70±0.58c 18.28±1.67b 23.05±0.37b 秋茶 1.39±0.16c 7.69±0.61b 13.16±0.66a 11.47±1.10a 12.63±1.40a GCG 春茶 0.06±0.01d 0.18±0.02c 0.23±0.07c 0.37±0.05b 0.47±0.05a 夏茶 0.04±0.02e 0.93±0.03c 0.79±0.06d 1.08±0.07b 1.25±0.01a 秋茶 0.20±0.04b 0.45±0.03b 0.45±0.16b 1.43±0.28a 1.53±0.08a GC 春茶 4.44±0.11a 2.50±0.19c 3.01±0.34b 0.47±0.04d 0.38±0.04d 夏茶 3.65±0.07a 0.92±0.11c 3.46±0.15a 0.83±0.04c 1.83±0.30b 秋茶 3.71±0.03a 1.03±0.11c 2.57±0.08b 0.53±0.05d 0.51±0.05d TECs 春茶 4.07±0.21d 38.43±1.01c 76.35±18.57b 75.12±1.03b 94.76±0.67a 夏茶 6.80±0.03e 56.53±1.28d 99.28±0.79b 88.52±3.78c 121.36±0.58a 秋茶 3.27±0.20e 41.83±3.34d 77.74±1.11b 67.05±2.64c 85.48±4.69a TSCs 春茶 7.80±0.21c 20.05±1.09b 17.50±1.44b 26.36±0.87b 22.52±1.96a 夏茶 6.67±0.21e 28.40±2.28c 15.55±0.87d 34.91±2.28b 48.18±1.92a 秋茶 6.84±0.21d 24.78±1.49b 14.89±0.38c 25.37±0.50b 28.49±1.01a TCs 春茶 11.87±0.38d 58.48±1.27c 93.85±17.19b 101.49±1.89b 117.27±2.23a 夏茶 13.46±0.24e 84.93±1.49d 114.83±0.87c 123.43±6.05b 169.54±2.40a 秋茶 10.10±0.22d 66.61±4.80c 92.63±1.03b 92.41±2.40b 113.97±5.71a 生物碱
Alkaloid/（mg·g−1）TB 春茶 0.48±0.09d 0.73±0.00c 0.91±0.03b 0.88±0.03b 1.52±0.11a 夏茶 0.34±0.02d 0.61±0.04c 1.32±0.11b 1.39±0.08b 1.65±0.08a 秋茶 0.34±0.03d 0.51±0.05c 1.17±0.06a 0.70±0.07b 1.25±0.04a CAF 春茶 16.16±1.12e 18.87±0.82d 33.39±0.38a 26.81±0.58c 31.96±0.20b 夏茶 13.56±0.60e 29.81±1.24d 39.69±1.24b 32.62±0.11c 42.81±0.92a 秋茶 9.84±0.55d 12.53±0.71c 30.88±1.56a 23.70±1.68b 24.26±0.65b 总量 春茶 16.64±1.21e 19.61±0.83d 34.30±0.37a 27.69±0.62c 33.47±0.22b 夏茶 13.90±0.62e 30.42±1.28d 41.01±1.35b 34.00±0.17c 44.46±0.97a 秋茶 10.18±0.55d 13.04±0.67c 32.06±1.62a 24.40±1.74b 25.51±0.69b
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表 3 不同茶季金牡丹5大类茶氨基酸含量
Table 3 Amino acid content of 5 types of Jinmudan tea of different seasons
氨基酸名称
Amino acid茶季
Tea season氨基酸含量
Amino acid content/（mg·g−1）红茶
Black tea乌龙茶
Oolong tea白茶
White tea黄茶
Yellow tea绿茶
Green tea苦味氨基酸
Bitter amino acids蛋氨酸 Met 春茶 0.047±0.009ab 0.037±0.005b 0.037±0.002b 0.041±0.003ab 0.049±0.007a 夏茶 0.022±0.000c 0.047±0.006a 0.037±0.006b 0.023±0.006c 0.040±0.000ab 秋茶 0.052±0.004a 0.041±0.003b 0.036±0.002bc 0.038±0.002bc 0.035±0.002c 精氨酸 Arg 春茶 0.505±0.089b 0.410±0.035b 0.959±0.108a 0.529±0.015b 0.533±0.038b 夏茶 0.283±0.054b 0.289±0.081b 0.275±0.030b 0.608±0.055a 0.519±0.154a 秋茶 0.462±0.016bc 0.450±0.000c 0.796±0.054a 0.432±0.009c 0.511±0.027b 赖氨酸 Lys 春茶 0.402±0.049c 0.300±0.019d 1.498±0.036a 0.676±0.024b 0.724±0.056b 夏茶 0.157±0.030c 0.171±0.024c 0.270±0.055ab 0.341±0.075a 0.224±0.047b 秋茶 0.598±0.164b 0.590±0.124b 0.866±0.000a 0.506±0.082b 0.64±0.022b 络氨酸 Tyr 春茶 0.729±0.120b 0.533±0.037c 1.044±0.127a 0.575±0.015bc 1.168±0.138a 夏茶 0.421±0.083b 0.263±0.043bc 0.615±0.127a 0.220±0.018c 0.678±0.135a 秋茶 0.514±0.060c 0.344±0.058d 1.309±0.095a 0.590±0.030c 1.156±0.034b 亮氨酸 Leu 春茶 0.390±0.056c 0.258±0.016d 0.464±0.033b 0.615±0.012a 0.666±0.033a 夏茶 0.717±0.123bc 0.036±0.006d 0.926±0.187a 0.610±0.009c 0.809±0.047ab 秋茶 0.368±0.057c 0.418±0.038c 0.690±0.083b 0.864±0.111a 0.442±0.109c 色氨酸 Trp 春茶 0.574±0.119b 0.563±0.059b 0.561±0.037b 0.590±0.029b 0.752±0.027a 夏茶 0.442±0.054bc 0.615±0.086ab 0.531±0.166bc 0.381±0.096c 0.745±0.045a 秋茶 0.362±0.071c 0.697±0.119a 0.705±0.061a 0.526±0.006b 0.696±0.029a 缬氨酸 Val 春茶 0.481±0.045c 0.401±0.041c 1.190±0.090a 0.726±0.018b 1.213±0.120a 夏茶 0.427±0.045c 0.244±0.082c 1.740±0.489a 0.925±0.084b 1.173±0.249b 秋茶 0.461±0.055c 0.768±0.134b 0.708±0.021b 0.805±0.111b 1.326±0.079a 异亮氨酸 Ile 春茶 0.703±0.075d 0.487±0.024e 0.871±0.054c 1.063±0.044b 1.320±0.102a 夏茶 0.357±0.067b 0.383±0.000b 0.719±0.022a 0.435±0.026b 0.485±0.133b 秋茶 0.677±0.092b 0.281±0.027c 0.635±0.295b 0.591±0.107b 1.232±0.063a 组氨酸 His 春茶 0.164±0.008c 0.205±0.022bc 0.251±0.02b 0.236±0.005b 0.353±0.048a 夏茶 0.094±0.031b 0.067±0.055b 0.155±0.085ab 0.159±0.020ab 0.220±0.015a 秋茶 0.081±0.021b 0.111±0.040b 0.150±0.092b 0.110±0.048b 0.356±0.010a 合计 春茶 3.994±0.554c 3.193±0.233d 6.875±0.457a 5.050±0.037b 6.778±0.548a 夏茶 2.919±0.184c 2.115±0.199d 5.268±0.643a 3.703±0.088b 4.893±0.189a 秋茶 3.576±0.336c 3.698±0.389c 5.896±0.467a 4.463±0.457b 6.392±0.266a 甜味氨基酸
Sweet amino acids丙氨酸 Ala 春茶 0.623±0.104b 0.613±0.063b 1.451±0.171a 0.595±0.066b 0.666±0.018b 夏茶 0.763±0.102a 0.449±0.057b 0.864±0.005a 0.548±0.086b 0.755±0.185a 秋茶 0.588±0.103c 0.807±0.047b 1.097±0.066a 0.824±0.068b 0.350±0.046d 脯氨酸 Pro 春茶 0.329±0.107b 0.202±0.053c 0.469±0.052a 0.347±0.021b 0.428±0.022ab 夏茶 0.483±0.119b 0.427±0.062b 0.678±0.026a 0.267±0.032c 0.417±0.088b 秋茶 0.689±0.032b 0.593±0.081b 0.839±0.123a 0.431±0.034c 0.259±0.033d 苏氨酸 Thr 春茶 0.571±0.024d 0.554±0.048d 0.708±0.056c 0.863±0.002b 1.095±0.112a 夏茶 0.487±0.082b 0.306±0.127b 0.899±0.108a 0.525±0.230b 1.100±0.051a 秋茶 0.537±0.084b 0.537±0.029b 0.828±0.128a 0.599±0.050b 0.878±0.064a 丝氨酸 Ser 春茶 1.544±0.151c 1.541±0.151c 2.576±0.047a 2.247±0.061b 2.203±0.113b 夏茶 0.896±0.094b 0.761±0.166b 1.600±0.365a 1.509±0.025a 1.475±0.154a 秋茶 1.121±0.147c 1.073±0.117c 2.143±0.082a 2.095±0.023a 1.831±0.121b 甜味氨基酸
Sweet amino acids合计 春茶 3.066±0.172c 2.910±0.269c 5.205±0.164a 4.052±0.145b 4.392±0.201b 夏茶 2.629±0.135b 1.943±0.065c 4.042±0.353a 2.849±0.332b 3.747±0.370a 秋茶 2.936±0.109d 3.010±0.165d 4.907±0.195a 3.949±0.096b 3.319±0.163c 鲜味氨基酸
Umami amino acids天冬氨酸 Asp 春茶 1.327±0.246b 0.763±0.105c 1.324±0.082b 0.515±0.103c 2.638±0.367a 夏茶 0.353±0.113b 0.064±0.054c 0.371±0.182b 0.190±0.091bc 1.047±0.021a 秋茶 2.305±0.121a 1.035±0.094b 0.934±0.060b 0.638±0.125c 2.291±0.076a 天冬酰胺 Asn 春茶 0.610±0.059c 0.655±0.071c 2.266±0.092a 1.473±0.079b 1.423±0.077b 夏茶 0.462±0.120c 0.179±0.044d 1.214±0.240a 0.791±0.068b 0.773±0.199b 秋茶 0.321±0.046c 0.388±0.065c 1.741±0.043a 1.202±0.103b 1.139±0.035b 茶氨酸 Thea 春茶 5.729±0.226e 14.017±0.135d 27.499±0.102a 15.203±0.572c 18.966±0.089b 夏茶 4.318±0.195c 9.552±0.389b 15.930±0.630a 8.850±0.354b 16.299±1.098a 秋茶 5.215±0.139d 11.824±0.532c 24.612±1.516a 11.068±0.072c 17.961±0.978b 谷氨酸 Glu 春茶 0.826±0.094d 1.081±0.083c 0.812±0.086d 1.410±0.053b 2.083±0.111a 夏茶 0.448±0.053d 0.762±0.037b 0.304±0.028e 0.601±0.057c 1.697±0.084a 秋茶 0.488±0.034d 0.683±0.050c 0.613±0.121cd 1.192±0.075b 1.591±0.026a 谷氨酰胺 Gln 春茶 0.856±0.033d 3.519±0.453a 0.942±0.048d 1.483±0.030c 2.117±0.191b 夏茶 0.540±0.053d 2.018±0.148a 0.553±0.037d 0.980±0.074c 1.864±0.034b 秋茶 0.500±0.037e 2.272±0.041a 0.821±0.009d 1.310±0.085c 2.057±0.021b 合计 春茶 9.349±0.358d 20.036±0.712c 32.844±0.271a 20.084±0.752c 27.227±0.800b 夏茶 6.120±0.231e 12.576±0.416c 18.372±0.828b 11.412±0.360d 21.680±0.954a 秋茶 8.829±0.209d 16.202±0.566c 28.721±1.568a 15.409±0.169c 25.039±1.062b 无味氨基酸
Odorless amino acidsβ氨基丁酸 β-ABA 春茶 0.375±0.017b 0.313±0.032c 0.732±0.041a 0.392±0.018b 0.418±0.036b 夏茶 0.433±0.035bc 0.140±0.048d 0.744±0.021a 0.368±0.017c 0.463±0.055b 秋茶 0.362±0.040b 0.243±0.035c 0.410±0.065ab 0.106±0.025d 0.448±0.018a γ-氨基丁酸 GABA 春茶 0.347±0.01bc 0.308±0.027c 0.506±0.049a 0.399±0.011b 0.462±0.031a 夏茶 0.247±0.027c 0.331±0.009bc 0.490±0.028a 0.277±0.092c 0.397±0.023b 秋茶 0.207±0.013c 0.268±0.035ab 0.345±0.056ab 0.233±0.115bc 0.369±0.060a 鸟氨酸 Orn 春茶 0.118±0.013b 0.158±0.030ab 0.216±0.035a 0.150±0.028ab 0.201±0.054a 夏茶 0.051±0.021b 0.071±0.014b 0.046±0.000b 0.055±0.022b 0.181±0.019a 秋茶 0.113±0.008a 0.069±0.022b 0.137±0.029a 0.066±0.013b 0.071±0.011b 合计 春茶 0.840±0.029d 0.779±0.067d 1.454±0.040a 0.941±0.049c 1.081±0.061b 夏茶 0.731±0.026c 0.541±0.047d 1.280±0.032a 0.700±0.081c 1.042±0.039b 秋茶 0.682±0.024b 0.580±0.017b 0.892±0.075a 0.405±0.089c 0.888±0.088a 总计 春茶 17.249±0.580e 26.918±1.225d 46.378±0.733a 30.127±0.713c 39.477±1.580b 夏茶 12.400±0.318e 17.175±0.409d 28.961±0.597b 18.664±0.127c 31.362±0.534a 秋茶 16.023±0.328d 23.491±0.772c 40.416±2.004a 24.226±0.267c 35.638±0.799b
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表 4 不同茶季金牡丹5大茶类TAV值
Table 4 The TAV value of 5 kinds of Jinmudan tea in different tea seasons
氨基酸名称
Amino acid阈值
Threshold/
(mg·g−1)茶季
Tea season滋味活性值
TAV value红茶
Black tea乌龙茶
Oolong tea白茶
White tea黄茶
Yellow tea绿茶
Green tea蛋氨酸 Met 0.30 春茶 0.15±0.03ab 0.12±0.02b 0.13±0.01ab 0.14±0.01ab 0.16±0.02a 夏茶 0.07±0.00c 0.16±0.02a 0.10±0.03bc 0.08±0.02c 0.13±0.00ab 秋茶 0.17±0.01a 0.14±0.01b 0.12±0.01bc 0.13±0.01bc 0.12±0.01c 精氨酸 Arg 0.50 春茶 1.01±0.18b 0.82±0.07b 1.57±0.45a 1.06±0.03b 1.07±0.08b 夏茶 0.57±0.11b 0.58±0.16b 0.8±0.47ab 1.22±0.11a 1.04±0.31ab 秋茶 0.92±0.03b 0.90±0.00b 1.35±0.42a 0.86±0.02b 1.02±0.05ab 赖氨酸 Lys 0.50 春茶 0.80±0.10bc 0.60±0.04c 2.42±0.92a 1.35±0.05bc 1.45±0.11b 夏茶 0.31±0.06b 0.34±0.05b 0.60±0.21a 0.68±0.15a 0.45±0.09ab 秋茶 1.20±0.33ab 1.18±0.25ab 1.56±0.31a 1.01±0.16b 1.28±0.04ab 络氨酸 Tyr 0.91 春茶 0.80±0.13bc 0.59±0.04c 0.96±0.32b 0.63±0.02c 1.28±0.15a 夏茶 0.46±0.09b 0.29±0.05b 0.49±0.25b 0.24±0.02b 0.75±0.15a 秋茶 0.56±0.07b 0.38±0.06b 1.21±0.49a 0.65±0.03b 1.27±0.04a 亮氨酸 Leu 1.90 春茶 0.21±0.03c 0.14±0.01d 0.27±0.05b 0.32±0.01a 0.35±0.02a 夏茶 0.38±0.06ab 0.02±0.00c 0.39±0.07ab 0.32±0.00b 0.43±0.02a 秋茶 0.19±0.03b 0.22±0.02b 0.4±0.11a 0.45±0.06a 0.23±0.06b 色氨酸 Trp 0.90 春茶 0.64±0.13b 0.63±0.07b 0.61±0.02b 0.66±0.03b 0.84±0.03a 夏茶 0.49±0.06b 0.68±0.10ab 0.54±0.25b 0.42±0.11b 0.83±0.05a 秋茶 0.40±0.08c 0.77±0.13a 0.72±0.14ab 0.58±0.01b 0.77±0.03a 缬氨酸 Val 0.40 春茶 1.20±0.11c 1.00±0.10c 2.52±0.64a 1.81±0.05b 3.03±0.30a 夏茶 1.07±0.11bc 0.61±0.21c 3.28±1.37a 2.31±0.21b 2.93±0.62a 秋茶 1.15±0.14c 1.92±0.33b 1.94±0.34b 2.01±0.28b 3.32±0.20a 异亮氨酸 Ile 0.90 春茶 0.78±0.08c 0.54±0.03d 1.04±0.17b 1.18±0.05b 1.47±0.11a 夏茶 0.4±0.07b 0.43±0.00b 0.69±0.18a 0.48±0.03ab 0.54±0.15ab 秋茶 0.75±0.10b 0.31±0.03c 0.78±0.30b 0.66±0.12b 1.37±0.07a 组氨酸 His 0.20 春茶 0.82±0.04c 1.02±0.11bc 1.2±0.01b 1.18±0.02b 1.77±0.24a 夏茶 0.47±0.16bc 0.34±0.28c 0.67±0.37bc 0.79±0.10ab 1.10±0.08a 秋茶 0.4±0.10c 0.56±0.20c 0.92±0.25b 0.55±0.24c 1.78±0.05a 丙氨酸 Ala 0.60 春茶 1.04±0.17b 1.02±0.10b 1.9±0.74a 0.99±0.11b 1.11±0.03b 夏茶 1.27±0.17ab 0.75±0.10c 1.32±0.21a 0.91±0.14bc 1.26±0.31ab 秋茶 0.98±0.17c 1.34±0.08b 1.70±0.32a 1.37±0.11b 0.58±0.08d 脯氨酸 Pro 3.00 春茶 0.11±0.04a 0.07±0.02b 0.15±0.03a 0.12±0.01a 0.14±0.01a 夏茶 0.16±0.04ab 0.14±0.02ab 0.18±0.07a 0.09±0.01b 0.14±0.03ab 秋茶 0.23±0.01a 0.2±0.03ab 0.22±0.08a 0.14±0.01bc 0.09±0.01c 苏氨酸 Thr 2.60 春茶 0.22±0.01c 0.21±0.02c 0.29±0.04b 0.33±0.00b 0.42±0.04a 夏茶 0.19±0.03c 0.12±0.05c 0.33±0.05b 0.20±0.09c 0.42±0.02a 秋茶 0.21±0.03b 0.21±0.01b 0.31±0.06a 0.23±0.02b 0.34±0.02a 丝氨酸 Ser 1.50 春茶 1.03±0.10c 1.03±0.10c 1.66±0.11a 1.50±0.04b 1.47±0.08b 夏茶 0.60±0.06b 0.51±0.11b 1.14±0.13a 1.01±0.02a 0.98±0.10a 秋茶 0.75±0.10c 0.72±0.08c 1.44±0.04a 1.4±0.02a 1.22±0.08b 天冬氨酸 Asp 1.00 春茶 1.33±0.25b 0.76±0.11bc 1.01±0.52bc 0.52±0.10c 2.64±0.37a 夏茶 0.35±0.11b 0.06±0.05c 0.31±0.23b 0.19±0.09bc 1.05±0.02a 秋茶 2.31±0.12a 1.04±0.09b 0.81±0.26bc 0.64±0.13c 2.29±0.08a 天冬酰胺 Asn 1.00 春茶 0.61±0.06c 0.66±0.07c 1.96±0.47a 1.47±0.08b 1.42±0.08b 夏茶 0.46±0.12b 0.18±0.04c 0.99±0.17a 0.79±0.07a 0.77±0.20a 秋茶 0.32±0.05c 0.39±0.06c 1.58±0.26a 1.2±0.10b 1.14±0.03b 茶氨酸 Thea 4.18 春茶 1.37±0.05c 3.35±0.03b 5.55±1.76a 3.64±0.14b 4.54±0.02ab 夏茶 1.03±0.05c 2.29±0.09b 3.23±1.06a 2.12±0.08b 3.90±0.26a 秋茶 1.25±0.03c 2.83±0.13bc 4.77±1.86a 2.65±0.02c 4.3±0.23ab 谷氨酸 Glu 0.30 春茶 2.75±0.31c 3.60±0.28c 3.51±1.16c 4.70±0.18b 6.94±0.37a 夏茶 1.49±0.18d 2.54±0.12b 1.30±0.47d 2.00±0.19c 5.66±0.28a 秋茶 1.63±0.11c 2.28±0.17c 2.46±1.10c 3.97±0.25b 5.30±0.09a 谷氨酰胺 Gln 0.50 春茶 1.71±0.07d 7.04±0.91a 2.23±0.68cd 2.97±0.06c 4.23±0.38b 夏茶 1.08±0.11c 4.04±0.30a 1.42±0.61bc 1.96±0.15b 3.73±0.07a 秋茶 1.00±0.07e 4.54±0.08a 1.90±0.45d 2.62±0.17c 4.11±0.04b
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1. 裘珍飞，曾炳山，范春节. 缺磷对黑木相思生长、营养和叶绿素荧光的影响. 中南林业科技大学学报. 2021(08): 9-17 . 
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