Objective   Full-length cDNA of Caffeoyl-CoA-O-methyltransferase gene (CCoAOMT) in the lignin metabolism pathway of soybean was cloned, and bioinformatics analyzed to study the resistance mechanism to cyst nematode (Heterodera glycines Ichinohe) of the plant.

  Method   CCoAOMT sequence from roots of a cyst nematode-resistant Huipizhiheidou soybean plant was cloned by RT-PCR.

  Results   The full length cDNA of GmCCoAOMT was cloned and then submitted to GenBank with the accession number of MW480860. The length of GmCCoAOMT was 848 bp, containing an ORF of 741bp and encoding 246 amino acids with a predicted molecular weight of 27.6 kDa and a theoretical isoelectric point of 5.67. It was located in the cytoplasm with a conserved AdoMet_MTases domain and belonged to that superfamily. The multiple alignment on amino acid sequence and phylogenetic tree indicated a high similarity between the gene and those of other leguminous plants.

  Conclusion   The cDNA of GmCCoAOMT was successfully cloned and shown with a close evolutionary relationship with Vigna unguiculata (Linn.) Walp., Cajanus cajan (Linn.) Millsp., Phaseolus vulgaris Linn. and Vigna angularis (Willd.) Ohwi et Ohashi but far from Lupinus albus and Cicer arietinum Linn.