西藏林芝市察隅县水稻根际土壤可培养酵母菌多样性及其与理化因子相关性

王艳红; 郝兆; 张艳茗; 郭小芳; 德吉

doi:10.19303/j.issn.1008-0384.2021.07.011

西藏林芝市察隅县水稻根际土壤可培养酵母菌多样性及其与理化因子相关性

Relationship between Environmental Factors and Diversity of Culturable Yeasts in Rhizosphere Soil of Rice Field on Tibetan Plateau

摘要

摘要:
目的开展林芝市察隅县水稻根际土壤可培养酵母菌多样性研究，并探究其与土壤理化因子的相关性。
方法采用稀释涂布法分离纯化酵母菌，并采用rRNA ITS区域序列分析与经典分类法对酵母菌菌株进行鉴定。采用R 3.6.1 分析可培养酵母菌多样性及其与土壤理化因子之间的关系。
结果从林芝市察隅县水稻根际土壤中共分离得到352株酵母菌，分属于10个属13个种，优势种为Cryptococcus podzolicus。Pearson相关系数显示，全氮和全磷与酵母菌种数及属数呈极显著正相关，全氮与酵母菌Shannon-Wiener多样性指数、Simpson多样性指数呈极显著正相关，与Pielou均匀度指数呈显著正相关，全磷与Shannon-Wiener多样性指数呈显著正相关；全氮与Candida pseudolambica、Goffeauzyma gastrica、Papiliotrema aspenensis以及Solicoccozyma terricola呈显著正相关；全磷与Goffeauzyma gastrica和Solicoccozyma terricola呈极显著正相关，与Candida pseudolambica呈显著正相关；含水量与Papiliotrema aspenensis呈极显著正相关。
结论不同土壤理化因子对水稻根际酵母菌的影响程度不同，其中全氮和全磷是影响水稻根际酵母菌群落结构的主要因素。

Abstract:
Objective Diversity of culturable yeasts in rhizosphere soil of rice fields at Zayü County, Nyingchi City, Tibet was determined and its correlation with the environmental factors analyzed.
Method Yeasts from the soil samples were isolated by dilution with a streaking plate method and identified by sequencing ITS domains of rRNA gene and conventional classification. Software R 3.6.1 was used to examine correlations between the yeast diversity and environmental factors.
Result A total of 352 yeast strains of 10 genera and 13 species dominated by Cryptococcus podzolicus were isolated. The numbers extremely significantly correlated with the contents of total nitrogen (TN) and total phosphorus (TP) in the habitat rhizosphere soil. TN extremely significantly correlated to both Shannon-Wiener and Simpson diversity indices and significantly correlated to Pielou evenness index, but TP only significantly correlated to Shannon-Wiener diversity index. At species level, the abundance of Candida pseudolambica, Goffeauzyma gastrica, Papiliotrema aspenensis, and Solicoccozyma terricola significantly correlated to TN, G. gastrica and S. terricola extremely significantly and C. pseudolambica significantly correlated to TP, and P. aspenensis extremely significantly correlated to the water content (WC) in soil.
Conclusion Different environmental factors affected differently on the rhizosphere yeast community on rice fields in the region. TN and TP in soil appeared to play a prominent role in that respect.

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