荔枝蝽<i>Tessaratoma papillosa</i>卵黄原蛋白及受体的序列及时空表达分析

程林; 韩顺财; 蒋敬涛; 李海超; 彭凌飞

doi:10.19303/j.issn.1008-0384.2021.07.009

荔枝蝽Tessaratoma papillosa卵黄原蛋白及受体的序列及时空表达分析

Sequences and Spatiotemporal Expressions of Vitellogenin and Vitellogenin Receptor Genes of Tessaratoma papillosa

摘要

摘要:
目的为荔枝蝽Tessaratoma papillosa的产卵繁殖行为提供分子层面的理论基础，并为荔枝蝽防治的靶点筛选提供有益思路。
方法采用转录组测序的方法，对荔枝蝽不同发育时期及组织进行转录组测序。通过筛选荔枝蝽的转录组数据和分子克隆的方法获得荔枝蝽卵黄原蛋白及其受体基因，并利用实时荧光定量PCR（qRT-PCR）分析其在不同发育阶段和组织部位的时空表达情况。
结果获得荔枝蝽3个卵黄原蛋白基因（T.papi_Vg1，T.papi_Vg2，T.papi_Vg3）和1个卵黄原受体蛋白基因（T.papi_VgRs）。对4个基因进行分析，发现其均具有典型的保守结构域，是典型的昆虫Vg和VgRs基因。从进化关系看，荔枝蝽的Vg和VgRs基因的分子进化与物种之间的进化关系较为匹配。qRT-PCR结果显示Vg1基因在雌虫各个组织中表达量都比较高，雄成虫中仅在淋巴液中表达量较高，而Vg2和Vg3基因仅在雌虫脂肪体中较高表达。VgRs的表达与Vg的表达部位基本一致，在雌虫卵巢中表达量最高，其次是雌虫、雄虫淋巴液和若虫的脂肪体当中，在若虫触角、雄虫触角和雄虫精巢中也有少量表达。
结论获得了荔枝蝽3个卵黄原蛋白基因和1个卵黄原蛋白受体基因，对其结构和进化关系进行探讨，并对其时空表达情况进行分析，为后续对荔枝蝽新防治靶标的筛选奠定基础。

Abstract:
Objective Molecular information associated with the oviposition and reproduction of Tessaratoma papillosa was studied for control of the pest on lychee trees.
Method Transcriptome sequencing of T. papillosa in various tissues and developmental stages was conducted. The vitellogenin and vitellogenin receptor genes were obtained by screening the transcriptome database and applying molecular cloning methods. Temporal and spatial expressions of the genes were analyzed using qRT-PCR.
Result Three vitellogenin genes (i.e., T. papi_Vg1, Vg2, and Vg3) and one receptor gene, T. papi_VgRs, were obtained after screening. Homologous analysis showed the genes contained conserved domains typical in the Vg and VgRs of insects. The molecular evolution of Vg and VgRs of T. papillosa paralleled that of the species. The temporal and spatial expressions of Vg1 shown by qRT-PCR were relatively high in all female tissues, but only the lymphatic fluid in adult males, whereas those of Vg2 and Vg3 highly expressed only in female adipose tissue. The expression of VgRs, as well as Vg, was highest in female ovary, followed by female, male lymph fluid, nymph adipose tissue, and minute in nymph antenna, male antenna, and male testis.
Conclusion The structures and evolution of the 3 vitellogenin genes and one vitellogenin receptor gene were analyzed. The information on the spatiotemporal expressions of the genes would aid target selection in study for the control ofT. papillosa.

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