Effect of Magnesium on Endogenous Hormones, Medicinal Material Yield, and l-Borneol Accumulation of Growing Blumea balsamifera Plants
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摘要:目的 明确施镁对生长期艾纳香内源激素、产量和有效成分l-龙脑积累的影响情况。方法 以1年生艾纳香种子苗为试验材料，用七水合硫酸镁提供镁元素，共设置4个镁水平，分别为0.0（CK）、1.5、15.0、150.0 mg·mL−1，采用酶联免疫吸附法分别测定艾纳香叶片赤霉素（GA3）、生长素（IAA）、脱落酸（ABA）、玉米素（ZR）等4种内源激素含量。使用电子秤测定艾纳香药材产量，利用GC测定生长期不同阶段艾纳香叶片有效成分l-龙脑百分含量，通过计算确定l-龙脑单株产量。结果 在处理第30 d、60 d和第90 d时，不同施镁水平显著增加了艾纳香中GA3、IAA、ABA和ZR等4种内源激素含量，显著增加了生长期艾纳香药材产量，其中1.5 、15.0 mg·mL−1镁水平影响效果最显著。在处理第60 d和第90 d时，不同施镁水平下艾纳香中l-龙脑百分含量差异极显著（P＜0.01），在处理第60 d时，CK组l-龙脑百分含量最低，较1.5、15.0 和150.0 mg·mL−1镁处理组分别显著降低了34.37%、35.48%和25.00%。在处理第90 d时，1.5 mg·mL−1镁处理组l-龙脑百分含量显著高于其他处理组，在处理第30 d和第120 d时，各处理间差异不具有统计学意义（P＞0.05）。在整个处理过程中，与CK相比，其他3个施镁水平均显著提高了l-龙脑单株产量（P＜0.05）。结论 在处理第30 d，不同施镁水平显著促进了艾纳香内源激素、产量和l-龙脑百分含量和单株产量的积累。在处理第60 d、90 d和第120 d时，1.5、15.0 mg·mL−1镁素的作用效果较明显，对提高艾纳香药材产量和品质有显著的促进作用。Abstract:Objective Effects of magnesium (Mg) application on the endogenous hormones, medicinal material yield, and active componentl-borneol accumulation of Blumea balsamifera (L.) DC. during growth periods were studied.Method One-year-old seedlings of B. balsamifera were treated with MgSO4·7H2O to provide the plants with Mg at levels of 0.0, 1.5, 15.0 or 150.0 mg·mL−1. Spanning across the growth periods of the plants, contents of gibberellic acid (GA3), auxin (IAA), abscisic acid (ABA), and zeatin (ZR) in leaf were determined by the enzyme linked immunosorbent assay; yield of medicinal material on an electronic scale; and, contents of l-borneol by GC. The combined total l-borneol in life cycle was calculated as the per plant yield of the active component.Result On the 30th, 60th, and 90th day after the treatments, the 4 endogenous hormones and medicinal material yield of the treated plants significantly increased. The greatest effects were observed at the application levels of 1.5 or 15.0 mg·mL−1. Sampled on the 60th and 90th day, the l-borneol contents in leaf significantly differed by the varied treatments the plants received (P<0.01). The lowest content showed up in the leaves tested on the 60th day after the 0.0 mg·mL−1 control treatment, while 1.5 mg·mL−1 had 34.37%, 15.0 mg·mL−1 applications rendered 35.48%, and 150.0 mg·mL−1 resulted in 25.00% on l-borneol. At the end of 90d, the l-borneol in leaf under 1.5 mg·mL−1 treatment was significantly higher than those under other treatments, but no statistically significant differences found among the treatments on the 30th or the 120th day (P>0.05). All Mg applications significantly increased the per plant yields on l-borneol (P<0.05).Conclusion The Mg applications significantly boosted the endogenous hormones, medicinal material yield, and l-borneol production of the individual B. balsamifera plant in 30 d. On the 60th, 90th, and 120th day, the applications at the rates of 1.5 and 15.0 mg·mL−1 seemed more apparent in promoting the yield of the medicinal material and the content of l-borneol than either control or the highest dosage.
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Keywords:
- magnesium /
- Blumea balsamifera /
- endogenous hormones /
- yield /
- l-borneol
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图 2 不同施镁水平对艾纳香中生长激素含量的影响
Figure 2. Effect of Mg applications on IAA content of B. balsamifera
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图 4 不同施镁水平对艾纳香中玉米素含量的影响
Figure 4. Effect of Mg applications on ZT content of B. balsamifera
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图 5 不同施镁水平对艾纳香药材产量的影响
Figure 5. Effect of Mg applications on medicinal material yield of B. balsamifera
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图 6 不同施镁水平对艾纳香中l-龙脑百分含量的影响
Figure 6. Effect of Mg applications on l-borneol content of B. balsamifera
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图 3 不同施镁水平对艾纳香中脱落酸含量的影响
Figure 3. Effect of Mg applications on ABA content of B. balsamifera
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