Abstract:
Objective Genetic relationship and diversity of Agaricus bisporus germplasms recently introduced to southern Fujian were studied to facilitate the identification, preservation, and breeding of the mushroom resource.
Method Both somatic incompatibility test and SSR molecular marker technology were used to determine the genetic diversity of 9 varieties of A. bisporus.
Result Somatic incompatibilities of the germplasms allowed the separation of 5 distinct groups by the presence or absence of antagonistic effect among them. Utilizing a similarity coefficient of 0.77, SSR analysis clustered the germplasms into Group 1 that consisted of HK, Group 2 that included 901 and A15, Group 3 that had Fumo 52, and Group 4 that comprised of As2796, W192, W2000, Fumo 58, and Fumo 38.
Conclusion From the results obtained by the somatic incompatibility test and SSR molecular marker technology, HK appeared to be remotely related to the other 8 germplasms, especially the domestic varieties. Hence, it could be a potential candidate for breeding purpose.
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