• 中文核心期刊
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  • 中国科技核心期刊
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印度梨形孢对植物生长调节剂在水稻上过量使用的缓解作用

Mitigating Ill-effect of Plant Growth Regulator Overuse on Rice Plants by Piriformospora indica

    摘要
  • 摘要:
      目的  植物生长调节剂过量使用会对植物的生长造成不利影响，严重情况下甚至抑制植物生长，而印度梨形孢（Piriformospora indica，简称Pi）是一种植物根部内生菌，能增强植物的抗逆能力，探究印度梨形孢对植物生长调节剂在水稻上过量使用的危害是否具有缓解作用可为缓解植物生长调节剂胁迫提供方法依据。
      方法  利用国际水稻营养液培养水稻，施加过量植物生长调节剂（2,4-D或PP333），水培15 d后，分别测量各组水稻株高、根长、根数、根系活力及叶绿素含量，观察叶片ROS染色情况，分析在印度梨形孢是否接种的情况下，2,4-D或PP333对水稻叶片中抗氧化酶（SOD、POD、CAT）的活性变化影响。
      结果  施加过量植物生长调节剂，水稻生长状况受到抑制，接种过印度梨形孢的水稻受影响较未接种小；+Pi、+2,4-D组与−Pi、+2,4-D组相比，株高增加26.02%，根长增加17.27%，根数增加30.77%，叶绿素含量增加64.71%，根系活力增加43.72%；+Pi、+PP333组与−Pi、+PP333组相比，株高增加36.79%，根系活力增加23.64%；接种印度梨形孢以及2,4-D或PP333处理，使水稻叶片中ROS含量增加；2,4-D或PP333处理使水稻SOD、POD、CAT活性升高，接种过印度梨形孢的水稻，抗氧化酶活性维持在正常水平。
      结论  印度梨形孢对植物生长调节剂在水稻上过量使用具有缓解作用。植物生长调节剂过量使用的情况下，接种印度梨形孢的水稻株高增高、根系发达，植株整体发育比未接种印度梨形孢的水稻良好，印度梨形孢可能通过恢复水稻活性氧系统平衡，一定程度上减少水稻受植物生长调节剂过量使用的抑制作用。

     

    Abstract:
      Objective  Potential of applying an endophyte in plant roots, Piriformospora indica (Pi), to mitigate the ill-effect of excessive usage of plant growth regulators on rice paddies was investigated.
      Method  Rice seedlings were hydroponically grown in a nutrient solution for 15 d. The height and chlorophyll content of the plants as well as the length, number, and vitality of the roots were monitored. ROS in the leaves was examined by a staining method. Effects of 2,4-D or PP333 treatment with or without Pi addition on the activities of antioxidant enzymes (i.e., SOD, POD, and CAT) in leaves were determined.
      Result  Excessive applications of plant growth regulator can adversely affect or even retard the growth of rice plants. This study revealed that if a rice plant was inoculated with Pi it could become less vulnerable to the stress. Comparing 2,4-D treatment without Pi addition, the seedlings grew 26.02% taller, the roots 17.27% longer, the root count 30.77% more, the chlorophyll content 64.71% higher, and the root vitality 43.72% greater with the Pi addition. In the Pi+PP333 group, the plants were 36.79% taller and 23.64% higher in root vitality than without the presence of Pi. The leaf ROS of the rice seedlings increased with Pi, 2,4-D or PP333 in the nutrient solution. Either 2,4-D or PP333 treatment enhanced the SOD, POD and CAT activities, but the Pi inoculation did not exert any significant differences on them.
      Conclusion  It appeared that P. indica could indeed alleviate the stress imposed on rice plants due to overuse of plant growth regulators. By inoculating Pi in the medium, the rice plants could overcome the interference by 2,4-D or PP333, grow well, and develop a robust root system. Restoration of the antioxidant system in rice by Pi might allow the plant to effectively mitigate the ill caused by over-exposure of plant growth regulators.

     

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