• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

辐射诱导水稻粒色突变体的农艺性状及营养品质分析

Agronomic Characteristics and Nutritional Quality of Colored Grains from Radiation-induced Rice Mutants

  • 摘要:
      目的  利用辐射诱变获得有色稻,考察辐射诱导的水稻粒色突变体农艺性状及营养品质变化,为有色稻育种提供优良种质资源。
      方法  以Co60-γ射线辐射扬稻6号(9311)干种子获得果皮颜色改变的突变体。将突变体进行自交选育并保留果皮颜色红色或黑色的理想单株直至水稻性状稳定。对性状稳定的突变体的农艺性状以及营养品质进行检测。
      结果  辐射诱变获得的2个粒色突变体R9311-01、R9311-02的糙米皮颜色分别为黑色和红褐色,R9311-01的平均穗长、R9311-02的千粒重与对照9311-CK有显著差异,其余的农艺性状无显著差异。在营养品质方面,R9311-01、R9311-02的脂肪含量、支链淀粉含量、黄酮含量、花色苷含量均极显著高于亲本9311-CK(P<0.01),而粗蛋白与总氨基酸则无显著差异;此外,R9311-01的赖氨酸含量显著高于9311-CK(P<0.05),其余的氨基酸(R9311-01、R9311-02的所有氨基酸)则无显著差异。
      结论  辐射突变获得的黑稻R9311-01、红稻R9311-02继承了原始亲本9311-CK的大部分农艺性状且提高了营养品质,为有色稻的育种以及遗传研究提供了优质材料。

     

    Abstract:
      Objective  Agronomic characteristics and nutritional quality of colored rice grains from the mutated plants germinated from the irradiated seeds were analyzed.
      Method  Dried rice seeds of Yangdao 6 (9311-CK) were irradiated by Co60 to self-cross breed stable mutants that bore grain hulls with reddish brown or black color. Agronomic traits and nutritional quality of the harvested grains were determined.
      Result  R9311-01 of black grains and R9311-02 of reddish brown grains were obtained through the radiation treatment and select breeding. The mean ear length of R9311-01 and the 1000-grain weight of R9311-02 differed significantly from those of 9311-CK, but not the other agronomic traits. Aside from crude protein and total amino acid, the contents of fat, amylopectin, flavonoids, and anthocyanins in the grains of the mutant strains were significantly higher than those of control. Except the significantly higher lysine content in the grains of R9311-01, all other amino acids in the rice of either strain deviated significantly from control.
      Conclusion  The grains of black R9311-01 and reddish brown R9311-02 mutant rice obtained by irradiation inherited most of the agronomic traits of the grains of their parents 9311-CK but showed an improved nutrient content. Consequently, the radiation treatment might provide a venue for breeding high quality rice varieties bearing colored grains.

     

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