辣椒脉斑驳病毒湖南分离物全基因组序列测定及分子特征

谭汝晴; 罗香文; 卜姗; 张宇; 张松柏; 张德咏; 刘勇

doi:10.19303/j.issn.1008-0384.2020.02.009

辣椒脉斑驳病毒湖南分离物全基因组序列测定及分子特征

Full Genomic Sequence and Molecular Characteristics of Hunan Isolate of Chilliveinal mottle Virus

摘要

摘要:
目的辣椒脉斑驳病毒（Chilli veinal mottle virus，ChiVMV）是东南亚茄科作物主产地主要病毒种类之一，严重危害辣椒等茄科作物的生产。测定ChiVMV的全基因组序列，分析其分子特征，可以明确该病毒的适应性进化以及对我国辣椒等茄科作物的潜在威胁提供科学基础。
方法以湖南疑似感染ChiVMV辣椒为样本，采用small RNA高通量测序结合RT-PCR测定病毒全基因组序列，利用Mega、RDP及DnaSP等生物学软件分析其分子特征。
结果 ChiVMV湖南分离物全长基因组序列为9 704 nt（不包含3'-A尾），与其他分离物的序列同源性为 84% ～ 94 %。系统发育分析表明，我国的ChiVMV聚类为一个亚簇，与其他国家和地区分离物不存在重组事件。基因的替换指数R=3.29，替换碱基类型主要是 C/T替换。
结论碱基替换突变可能是ChiVMV湖南分离物适应性进化的主要因素。

Abstract:
Objective Characteristics and full genomic sequence of Chilliveinal mottle virus (ChiVMV), one of the most recurring viral pathogens infecting chili pepper plants in South-East Asia were studied to decipher the evolution of the virus for improved estimation on its potential threat to Solanacae crops.
Method The ChiVMV gene from Hunan isolate (ChiVMVhn) was sequenced using the small RNA high-throughput sequencing and RT-PCR. Molecular characteristics of the gene were analyzed by Mega, RDP, and DnaSP software.
Result The gene of ChiVMVhn contained 9,704 nucleotides (excluding 3’-poly A) and shared 84%-94% identity with those from the other ChiVMVs. The phylogenetic analysis suggested that ChiVMVhn represented an evolutionary isolate distinctive from those reported previously elsewhere in the world. The nucleotide substitution in ChiVMVhn gene was R=3.29 and dominated by C/T.
Conclusion RNA mutation, rather than recombination, in the genes was seen crucial in the evolution of ChiVMVhn. Further studies on varieties of ChiVMV would considerably aid the scientific understanding on the evolution and pathogenic potential of the virus.

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