Abstract:
Objective Effects of 3 plant growth regulators on the germination of black wheat seeds under salt-stress were studied to provide possible relief for the wheat planting in saline-alkali areas.
Method Using a paper bedding method, 1-N aphthaleneacetic acid (NAA), gibberellic acid (GA3) or 6-benzylamino-purine (6-BA) was applied on seeds of black wheat (Zimai 1) to observe the germination under varied salt concentrations.
Result The best rate, potential and other indices on the seed germination were found when 150 mg·L-1 NAA, 200 mg·L-1 GA3 or 5 mg·L-1 6-BA was used without salt-stress. The presence of NaCl:Na2SO4=1:1 significantly inhibited the germination. Addition of the plant growth regulators in different concentrations relieved the inhibitive effect to a certain extent. Both GA3 at 200 mg·L-1 and 6-BA at 5 mg·L-1 significantly alleviated the germination inhibition when medium to low salt-stress (i.e., 40-80 mmol·L-1) was imposed on the seeds. The germination tendency and rate were significantly decreased with increasing salt-stress. When the salt concentration was raised to 120 mmol·L-1, only the application of 6-BA 5 mg·L-1 exhibited an effect on the germination.
Conclusion The treatment of GA3 200 mg·L-1 or 6-BA 5 mg·L-1 could protect the black wheat seeds from being permanently harmed by a medium-to-low salt-stress and improve the germination.