• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

PEG模拟干旱胁迫对水稻抗氧化酶基因表达的影响

Expression of Antioxidant Enzyme Genes in Rice under PEG-simulated Drought-stress

    摘要
  • 摘要:
      目的  干旱是影响水稻生产的重要环境因素之一，在干旱条件下水稻植株体内会发生一系列的抗逆反应，其中参与防御反应的关键酶基因表达会发生明显的变化。因此，本研究拟分析干旱胁迫处理后抗氧化酶类基因的表达变化，为进一步研究水稻抗旱机制提供理论参考。
      方法  采用质量体积比为0（CK）、18%、20%、22%、24%、26%的聚乙二醇（PEG6000）对三叶一心期的籼稻航2号植株进行干旱胁迫处理，筛选适合处理籼稻航2号的PEG6000质量体积比；进一步采用PEG6000对航2号植株进行干旱胁迫处理，分别于处理0、2、4、8、12、24、48、72 h取样；并用SYBR Green I荧光定量PCR（qRT-PCR）分析PEG6000处理不同时间段后植株中抗氧化酶类基因表达，包括过氧化氢酶（CATA、CATB、CATC）、过氧化物酶（POX5.1、POX1）、超氧化物歧化酶（plastidic Cu/Zn-SOD，cytosolic Cu/Zn-SOD）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）基因的表达变化。
      结果  根据表型观察和植株存活率，筛选出籼稻航2号对PEG6000的耐受临界质量体积比为22%；qRT-PCR结果表明PEG6000胁迫处理后9个基因的表达均出现上调，大部分基因表达都呈先上调后下调的趋势，且一般PEG处理4 h之后基因表达出现较明显上调，说明这些基因均不同程度地参与了PEG胁迫反应；其中，过氧化氢酶A基因（CATA）表达变化最显著，处理8 h表达量上调至处理0 h的28倍。
      结论  PEG6000胁迫处理后主要的抗氧化酶类基因表达发生了明显的变化。

     

    Abstract:
      Objective  Expression of antioxidant enzyme genes of rice in response to drought-stress was studied.
      Method  Simulated drought conditions using PEG6000 on Indica rice Hang 2 were used for the experimentation. The plants at 3-leaf stage were initially treated with 0% (CK), 18%, 20%, 22%, 24% or 26% PEG6000 to determine the appropriate concentration for the subsequent test. Under the selected PEG6000 treatment level, plant samples were collected at 0, 2, 4, 8, 12, 24, 48 and 72 h for analysis. The expressions of antioxidant enzyme genes (i.e., CATA, CATB and CATC), peroxidase genes (i.e., POX5.1 and POX1), superoxide dismutase genes (i.e., plastidic Cu/Zn-SOD and cytosolic Cu/Zn-SOD), ascorbate peroxidase gene (i.e., APX), and glutathione reductase gene (i.e., GR) of the rice plants were determined by qRT-PCR.
      Result  Based on the phenotype and survival rate of the rice plants in the preliminary test, 22% PEG6000 was chosen for the simulation experiment. The results of qRT-PCR showed that all 9 genes were upregulated initially under the treatment but downregulated afterward. Most of the genes significantly upregulated 4 h after treatment showing a response of the genes to the stress. In particular, CATA exhibited a most significant change at 8 h which was 28 times of that at 0 h.
      Conclusion  The expression of antioxidant enzyme genes significantly reacted to the PEG6000 treatment.

     

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