Abstract: Yunyan 87 tobacco was used in a pot experiment to study the effect of an application of bamboocharcoal and a vinasse fertilizer on the growth of the plants in a soil artificially laden with quinclorac. The herbicide stress would normally cause a phytotoxicity showing physiological, ultrastructural and chemical composition abnormalities on the plants. The results showed that caused tobacco plant deformity growth by quinclorac residue in the soil. However, by adding bamboo charcoal and 100 g vinasse fertilizer per plant, the best mitigation results were observed. After transplanting, the tobacco seedling experienced on plant height and leaf width had significantly mitigation effect in 30 and 60 days. The roots developed well the length of the main roots longer than that of control (singnificant at P < 0.05). Bamboo charcoal and vinasse fertilizer addition in soil on activity of superoxide dismutase(SOD) singnificantly increased, and those of peroxidase(POD) and malondialdehyde(MDA) singnificantly decreased in the tobacco leaves. Meanwhile, the number of starch granules in the leaf chloroplasts increased, the cell walls became clear, the plastids adhered tightly to the cell wall, and the cell structure of some previously damaged organelles mended. The chemical composition of tobacco leaves was improved, with a nitrogen/alkali ratio of 0.97 and suger/alkali ratio of 7.01.