Abstract: Molecular identification,variation, and phylogeneticity of the yellow leaf curl virus (TYLCV) on tomato plants in Fujian were investigated. For the study, specific primers were designed according to the known genome sequence of TYLCV for PCR detection. Then, the generated specific segments were collected, cloned and sequenced. A 541 bp fragment was obtained and its nucleotide sequence showed a high homogeneity, i.e., 97%-99%, with that of the tomato yellow curl leaf virus isolated from the domestic as well as international origins. It confirmed the presence of TYLCV infection on tomato plants in 5 cities in the province, and disease prevention and control measure should soon be implemented.