Abstract: To analyze the pollen vitality and hybrid pod setting ability of Oncidium,20 varieties were used as materials,2,3,5-Triphenyl Tetrazolium Chloride(TTC)was applied to determine the pollen vitality,and mating combinations were prepared for hybridization pollination experiments.The results showed that the highest pollen vitality of Oncidium was occurred when the day flowers blooming.Pollen vitality performed differently between the varieties and 16 varieties showed pollen vitality with range from 2.00% to 72.33%.The varieties with pollen dynamic were used as male parent,27 hybrid combinations were prepared,the 14 of them were mature in fruit with pod setting rate from 8.33%to 94.83%.The rate of pod setting was higher in interspecific hybridization(70.00%)than in intergeneric hybridization(41.18%).