Abstract: The effects of Bacillus Subtilis on muscle nutrition of Philippine eel,Anguill marmorata were investigated.Analysis of the amino acid composition and fatty acid composition of Anguill marmorata fed with Bacillus Subtilis showed no significant differences in moisture content,protein content and fat content among 4groups(P>0.05).Anguill marmorata is a high-quality protein source according to FAO/WHO model,which contains Glu with the maximum content,and followed by Asp,Arg and Lys,while Bacillus Subtilis showed no significant effect on amino acid composition of muscle of Anguill marmorata(P>0.05).All these amino acids are helpful for recovery,and also make up for lack of Lys in grain.,Muscle of Anguill marmorata contains more than11 kinds of fatty acid,including 3kinds of saturated fatty acid,4kinds of monounsaturated fatty acid and 3kinds of polyunsaturated fatty acid.Meanwhile,Anguill marmorata is rich in unsaturated fatty acid(63.2%,65.2%).When the Bacillus Subtilis supplemental level was between 1% to 3%,the MUFA and PUFA content were enhanced significantly(P<0.05).