Abstract: Avian leukosis of laying hen caused serious harm in breeding industry in our province.To investigate the genetic evolution of subgroup J avian leucosis viruses(ALV-J)isolated from the local layer flocks in Fujian,the gp85 gene of three isolates from different farms in Fujian were sequenced and analysis.By comparing with the gp85 gene of the 18 reference strains from both at home and abroad in GenBank,the results showed that the gp85 gene of these three were highly related with domestic layer isolates subgroup II(including CL09DP02,GL09DP01 and HuB09JY03)and the prototype ALV-J strain HPRS-103(97.8%-99.6%),indicating that the layer ALV-J isolates in Fujian may have the same origination with part of the domestic layer isolates.