Abstract: Three different temperatures(6,12and 20℃)and four culture mediums(lixivium of shrimp flesh, lixivium of wheat,beef extract and peptone culture solution)were used to reveal the population dynamics of P.persalinus in different conditions.The results showed that:the proliferation rate of the population density at 6℃ is slow,increased with the concentration of culture media,reached growth period at day 9and got the maximum on day 12;at 20℃,the population density increased rapidly and the group of beef extract reached the maximum population density(57 490ind·mL-1)on day 3;at 12℃,the population density showed:beef extract> wheat lixivium >shrimp flesh lixivium> peptone.The largest population density of the optimal concentration of culture solution at 12℃showed:beef extract(2.25g·L-1)> shrimp flesh(4.5g·L-1)>wheat(10.14g·L-1) > peptone(2.25g·L-1);the population growth rates showed:beef extract(2.25g·L-1)>wheat(10.14 g·L-1)>shrimp flesh(4.5g·L-1)> peptone(2.25g·L-1),therefore,in lixivium of shrimp flesh and wheat the population density was bigger and then the stationary phase was significantly longer than other culture solutions.Our results suggested that beef extract conld be applied to rapidly expanding growth of short-term population,the lixivium of shrimp flesh and wheat conld be applied to laboratory breeds conservation,and the population density and population growth rate could reach the maximum at 12℃ and 20℃,respectively.These findings will be benefit for the providing of a laboratory culture and preservation method of P.persalinus,and provide experimental ecological data of P.persalinus in marine aquaculture also.