福建部分沿海地区真鲷虹彩病毒病初步调查及基因进化分析
Preliminary Investigation and Phylogenetic Analysis of Red Sea Bream Iridoviral Disease(RSIVD)in Some Coastal Areas of Fujian Province
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摘要: 采集鱼样品25批, 利用PCR检测方法对我国福建部分沿海地区真鲷虹彩病毒病 (RSIVD) 情况进行初步调查, 对调查阳性结果的PCR扩增片段和阳性病毒株MCP基因PCR扩增片段进行测序分析, 同时, 与GenBank的虹彩病毒科各个属MCP全基因代表株进行同源性比较。初步调查结果表明, 我国福建部分沿海地区共检出2批RSIVD阳性样品, 受感染鱼种为大黄鱼和斜带髭鲷, 将获得的大黄鱼源虹彩病毒、斜带髭鲷虹彩病毒分别命名为RISV-FJ-DHY和RISV-FJ-XDZD;通过基因全序列比对和绘制同源树, 结果显示, 分离的2例病毒株均属于虹彩病毒科细胞肿大病毒属, RISV-FJ-DHY与2002年分离的大黄鱼病毒株发生了一定的变异, 而RISV-FJ-XDZD遗传性状稳定。Abstract: To investigate the infection of red sea bream iridoviral disease (RSIVD) , 25batches of fish collected from coasta1areas of Fujian Province were examined by PCR method, the positive amplified fragments and MCP gene of positive virus strains were sequenced and matched with the reported Iridoviridae MCP gene in Genbank.The result showed that two RSIVD strains was detected in samples from large yellow croaker and hapalogenys nitens respectively, which were named RISV-FJ-DHY and RISV-FJ-XDZD and found belonging to Megalocytivirus genus of Iridoviridae.Mutations were found in RISV-FJ-DHY and the strains isolated from large yellow croaker in 2002, while RISV-FJ-XDZD was genetically stable.
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