Time-dose-mortality Model Analysis of Beauveria bassiana XJBb3005 Against Myllocerinus aurolineatus
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摘要: 用浸虫法测定球孢白僵菌XJBb3005菌株对茶丽纹象甲成虫的致病力, 结果表明, 茶丽纹象甲成虫的死亡率随着球孢白僵菌孢子浓度的增加和作用时间的延长而增大, 当孢子浓度为1.0×108个孢子·mL-1, 处理7d后, 累计死亡率达100%。运用时间-剂量-死亡率模型 (time-dose-mortality, TDM) 对死亡率随时间、剂量的变化进行了拟合, 所建模型顺利通过Hosmer-Lemeshow拟合异质性检验, 表明模型的拟合良好, 并由此模型估计出了球孢白僵菌XJBb3005菌株对茶丽纹象甲成虫的致死剂量和致死时间。接种后第7d, LC50估计值为4.86×104孢子·mL-1, LC90估计值为4.26×106孢子·mL-1。用浓度为1.0×108、1.0×107、1.0×106、1.0×105和1.0×104个孢子·mL-1菌液处理的LT50值分别为3.97、4.45、5.01、6.49和7.87d。孢子悬液浓度为1.0×108、1.0×107和1.0×106孢子·mL-1处理时, LT90值分别为4.98、6.48和7.84d。由此表明该球孢白僵菌菌株对茶丽纹象甲的生物防治具有较好的应用潜力。
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关键词:
- 球孢白僵菌 /
- 茶丽纹象甲 /
- 致病力 /
- 时间-剂量-死亡率模型
Abstract: The pathogenicity of Beauveria bassiana strain XJBb3005on the adult of Myllocerinus aurolineatus was evaluated by using the adult immersion assay.The results showed that the mortality of M.aurolineatus increased with the concentration of the conidia and immersion time, and 100% mortality is caused by the applying of 108conidia/mL on day 7.A time-dose-mortality model was employed to analyze the bioassay data and the model fitted the data well, resulting in parameters for estimating the time and dose effects.The estimated LC50and LC90value were 4.86×104and 4.26×106 conidia/mL on day 7, respectively.At the immersion concentrations of 1.0×108, 1.0×107, 1.0×106, 1.0×105 and 1.0×104conidia/mL, the estimated LT50values were 3.97, 4.45, 5.01, 6.49and 7.87d, respectively;the LT90values were 4.98, 6.48and 7.84dat concentrations of 1.0×108, 1.0×107 and1.0×106 conidias/mL, respectively.These results suggest that B.bassiana XJBb3005is benefit for the biological control of M.aurolineatus. -
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