Abstract: In the present study, the prey preference of the predatory mite Amblyseius eharai to the new eggs (yellow) and old eggs (brown) of Bemisia tabaci was examined.Our results showed that the predation efficiency of adult female A.eharai to the new eggs of B.tabaci is higher, the mean daily consumption are 8.6 (new) and 2.2 (old) eggs/day when the new/old eggs was supplied only, and the A.eharai preyed the new ones when the new/ old eggs was supplied simultaneously.Furthermore, the consumption by the adult female A.eharai increased along with the increasing of the predation density, and the predation efficiency increased along with the temperatures (16℃to 28℃) .The predation efficiency of A.eharai at 32℃ was higher than that at 20℃or 16℃, and was lower than that at 24℃ or 28℃.In addition, the highest rate of discovery of prey (a = 0.8054) and the shortest handling time (Th =0.0227) were obtained at 28℃.