Physiological and Biochemical Characteristics of Artificially Aged Rapeseeds
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摘要: 以油菜品种‘镇油6号′种子为材料,研究油菜种子在高温(50℃)高湿(相对湿度100%)老化处理后的萌发特性、种子浸出液相对电导率、丙二醛、可溶性蛋白和可溶性糖含量以及抗氧化酶活性的变化。结果表明,随着老化时间的增加,油菜种子的发芽势、发芽率、发芽指数及其活力指数均显著降低。同时,随着老化天数的延长,浸出液电导率显著性增大,浸出液可溶性糖含量则逐渐升高;可溶性蛋白含量表现出下降趋势;种子MDA含量呈现先升高后逐渐下降的趋势。此外,在油菜种子老化进程中,SOD、POD、CAT活性变化的趋势基本一致,其活性均随老化天数的延长而逐渐降低;研究发现,在油菜种子老化处理中,种子浸出液电导率、可溶性蛋白含量和可溶性糖含量与种子老化及劣变程度显著性相关;膜脂过氧化作用也可能是引起或加剧种子老化劣变的重要原因之一。Abstract: Using seeds of Zhenyou No.6,we studied the germination characteristics,relative electric conductivity,MDA content,soluble protein and soluble sugar,as well as the antioxidant-related indexes of the seeds in response to the artificial aging at 50℃ and RH 100%.The results showed that(a)the seed′s moisture content increased considerably in the course of the aging;(b)the rate,energy,index and vigor of the seed germination decreased with aging;(c)the relative electrolyte leakage and soluble sugar content in the seeds increased,while the soluble protein decreased;(d)the seed′s MDA content increased initially and followed by a decline;and(e)upon aging,SOD,POD and CAT activities in the seeds decreased significantly.In conclusion,during aging,the relative electrolyte leakage,soluble protein and soluble sugar contents of the seeds positively correlated with the its vigor index.It was speculated that the deterioration of the rapeseeds as they aged was induced by the increased peroxidation of the membrance lipids.
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Keywords:
- Rapeseed /
- artificial aging /
- seed deterioration
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