双孢蘑菇As2796全长cDNA文库的构建及鉴定

Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796

摘要: 双孢蘑菇As2796是我国的主栽品种。为方便获得相关的目的基因,构建了该菌株的全长cDNA文库,文库初始库容达3.3×106 cfu.mL-1,插入片段长度范围0.7~4.0kb,平均长度约1.5kb,重组率约82%,全长率约35%。文库扩增出了5个目的基因全长编码序列(CDS),表明该文库的有效性,今后可用于扩增所需的双孢蘑菇基因的全长cDNA或CDS。

Abstract: A.bisporus As2796 is the main cultivar in China.For easily cloning the related target genes,the full-length cDNA library of As2796 was constructed.The library had a content of 3.3×106 cfu·mL-1,an insertion range of about 0.7-4.0 kb,an average cDNA length of 1.5 kb,a recombination efficience of about 82%,and full-length ratio of about 35%.The coding sequences(CDS) of 5 target genes were got from the library by PCR amplification,which proved the validity of the library for amplifying full-length cDNA or CDS of A.bisporus genes in need.