猪传染性胃肠炎病毒福建株的分离鉴定

Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain

摘要: 采集福建省某猪场爆发的疑似病毒性腹泻的病死仔猪病料,利用ST细胞从病死仔猪空肠及肠系膜淋巴结材料中分离获得1株病毒,对该病毒进行快速金标检测、电镜形态学观察、中和试验及RT-PCR鉴定后,证实该分离株为TGEV,命名为TGEV福建分离株(TGEV-FJ)。该分离毒在ST细胞上的毒价约为10-6.75.(100μL)-1。TGEV-FJ株N基因全长1 149bp,编码382个氨基酸,将该基因核苷酸序列和推导的氨基酸序列与GenBank中TGEV参考毒株进行比较分析,两者核苷酸的同源性为95.4%~99.8%,氨基酸同源性为96.1%~100%。

Abstract: Samples were collected from dead piglets from a swine farm suffered from severe diarrhea in Fujian,2007.To determine the causes of the disease,a virus was isolated from mestenteric lymphnodes of the piglets with ST cells.Obvious CPEs had shown that this virus could proliferate well in ST cells.TCID50,by Reed-Muench,was 10-6.0·(100 μL)-1.The virus was identified to be TGEV with neutralization test,colloidal-gold strips,electronic microscopy and TGEV N gene(1 149 bp) RT-PCR.In comparison with publicated TGEVs in GenBank,homology of nucleotide and amino acid were 95.4% to 99.8% and 96.1% to 100%,respectively.The result indicated that the isolated strain is TGEV,which was first isolated in Fujian,and named as TGEV-FJ strain.