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Volume 19 Issue 3
Apr.  2012
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Article Contents
GUO De-zhang, YAN Zheng, LAI Zhong-xiong, LIN Qing-liang, WANG Jia-fu. Effects of different current field condition on the symmetrical fusion of cotyledonary protoplasts between Cucummis sativus L. and Cucurbita ficifolia Bouche[J]. Fujian Journal of Agricultural Sciences, 2004, 19(3): 181-184.
Citation: GUO De-zhang, YAN Zheng, LAI Zhong-xiong, LIN Qing-liang, WANG Jia-fu. Effects of different current field condition on the symmetrical fusion of cotyledonary protoplasts between Cucummis sativus L. and Cucurbita ficifolia Bouche[J]. Fujian Journal of Agricultural Sciences, 2004, 19(3): 181-184.

Effects of different current field condition on the symmetrical fusion of cotyledonary protoplasts between Cucummis sativus L. and Cucurbita ficifolia Bouche

  • Received Date: 2003-08-23
  • Publish Date: 2004-09-15
  • The studies of symmetrical fusion of protoplasts isolated from Cuixiu mini cucumber (Cucummis sativus L. ) and fig-baf gourd (Cucurbita ficifolia Bouche) were carried out. The results indicated that the conditions could lead to the best effect of cell fusion were using 0.5 mol·L-1 mannitol as fusional solution and setting the alternating current field intensity at 25 V·cm-1, the alternating current frequency at 2000 kHz, the direct current intensity at 200 V·cm-1, the direct current pulse width at 40μs and 3 direct pulse times. After cultured in shallow-layer liquid medium, large numbers of callus were regenerated. The genetic identification of callus and the plant regeneration were also evaluated.
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