• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 20 Issue 2
Apr.  2012
Turn off MathJax
Article Contents
LIN Cong-fa, ZHONG Ai-qing, WEI Ze-ping, LUO Yang-fen, WANG Shao-hua. Study on in vitro culture and rapid propagation of Pseudostellaria heterophylla growing point[J]. Fujian Journal of Agricultural Sciences, 2005, 20(2): 113-117.
Citation: LIN Cong-fa, ZHONG Ai-qing, WEI Ze-ping, LUO Yang-fen, WANG Shao-hua. Study on in vitro culture and rapid propagation of Pseudostellaria heterophylla growing point[J]. Fujian Journal of Agricultural Sciences, 2005, 20(2): 113-117.

Study on in vitro culture and rapid propagation of Pseudostellaria heterophylla growing point

  • Received Date: 2003-11-07
  • Rev Recd Date: 2004-02-03
  • Publish Date: 2005-06-15
  • The regeneration plants were acquired by culturing in vitro the growing points (in 0.2-0.5 mm length) of Pseudostellaria heterophylla.The orthogonal experiments and single factor experiments showed that the optimum medium for calli induction and shoot buds from calli was MS+6-BA 2 mg·L-1+NAA 0.2 mg·L-1 + GA30.1-0.2 mg·L-1+sugar 30 g·L-1.The medium suitable for calli subculture was MS+6-BA 1.5 mg·L-1 + NAA 0.01 mg·L-1+sugar 30 g·L-1.The plantlets’propagation had been done by 2 ways i.e.continuous calli differentiation and plantlets cuttage propagation.Basic MS medium was suitable for plantlets cuttage propagation.Medium MS (pH 6.0) containing 60 g·L-1 sugar was suitable to induce micro-root tuber, under the condition of 20℃ and 16 hours of light everyday.Medium MS containing 30 g·L-1 sugar was suitable for roots differentiation.The survival rate of the tissue culture plantlets exposed to air for 10-15 days reached 98.6% in the sandy soil under the condition of 15-20℃ and the relative moisture more than 90%.The mosaic disease rate of virus-free roots was 94.75 % lower than that of conventional culture system in the field.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1949) PDF downloads(446) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return