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LIN Zhi-min, CHEN Zai-jie, HU Tai-jiao, HU Chang-quan, YAN Jing-wan, SU Jun, WANG Feng. Expression and purification of rice codon optimized His-cry1Ca fusion protein in E.coli and preparation of polyclonal antibody against cry1Ca[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 24-28.
Citation:
LIN Zhi-min, CHEN Zai-jie, HU Tai-jiao, HU Chang-quan, YAN Jing-wan, SU Jun, WANG Feng. Expression and purification of rice codon optimized His-cry1Ca fusion protein in E.coli and preparation of polyclonal antibody against cry1Ca[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 24-28.
LIN Zhi-min, CHEN Zai-jie, HU Tai-jiao, HU Chang-quan, YAN Jing-wan, SU Jun, WANG Feng. Expression and purification of rice codon optimized His-cry1Ca fusion protein in E.coli and preparation of polyclonal antibody against cry1Ca[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 24-28.
Citation:
LIN Zhi-min, CHEN Zai-jie, HU Tai-jiao, HU Chang-quan, YAN Jing-wan, SU Jun, WANG Feng. Expression and purification of rice codon optimized His-cry1Ca fusion protein in E.coli and preparation of polyclonal antibody against cry1Ca[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 24-28.
Rice codon optimized cry1Ca gene was cloned into expression vector pET-28b(+) by means of restricted enzymatic digestion.His-cry1Ca fusion protein was highly expressed by SDS-PAGE analysis accumulating more than 24% of the total bacterial proteins.The main expression production was deposited as inclusion body.His-cry1Ca was then purified with Ni-NTA after treatment of solvents.The target protein was used as the antigen to immunize rabbits.ELISA assay showed that the titer of the prepared polyclonal antibody was 1:50000,and had high specificities.
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