• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 20 Issue 3
Apr.  2012
Turn off MathJax
Article Contents
HUANG Jia-quan, YIN Li-yan, SUN Zhong-hai. SEM and TEM observation of long-time in vitro cultured citrus cells after embryogenesis inducing treatment[J]. Fujian Journal of Agricultural Sciences, 2005, 20(3): 189-193.
Citation: HUANG Jia-quan, YIN Li-yan, SUN Zhong-hai. SEM and TEM observation of long-time in vitro cultured citrus cells after embryogenesis inducing treatment[J]. Fujian Journal of Agricultural Sciences, 2005, 20(3): 189-193.

SEM and TEM observation of long-time in vitro cultured citrus cells after embryogenesis inducing treatment

  • Received Date: 2004-12-16
  • Publish Date: 2005-09-15
  • Scanning electron microscopic and transmission electron microscopic observation on long-term-cultured callus of Citrus sinensis cv.Valencia were carried out.The results showed that after the callus incubated on MT basic medium for 20 days, the cells were closely linked, a few fibrils appeared on the rumpled surface of the cells, abundant starch granules and organelles were observed in the cells.When cell incubated on the MT basic medium for 50 days, the surface of the cells rumpled, but the fibrils disappeared, ribosome in the cytoplasm increased but the size of starch granules reduced.The callus incubated in the somatic embryo inducing medium for 20 days led to the formation of protuberance and fibrils, sometimes the fibrils formed network, cytoplasm became a mass, and no obvious organelles, starch granules and plasmodesmata were observed) after 50 days, the fibrils on the surface of the cell disappeared, the bulk of the cells and the gap between them increased, organelles and nucleolus reformed, the ribosome were abundant and formed polyers.
  • loading
  • [1]
    崔凯荣,邢更生,周功克,等.体细胞胚发生的生化基础[J].生命科学,2001,13(1):28-33.
    [2]
    Murashige T, Tucker D P H.Growth factor requirements of citrus tissue culture [C].Proc First Int Citrus Symp, 1969,3:1155-1161.
    [3]
    邢更生,邢更妹,崔凯荣,等.枸杞胚性细胞分化的超微结构研究[J].电子显微学报,1999,18(4):395-400.
    [4]
    王亚馥,崔凯荣,汪丽虹,等.小麦体细胞胚发生的超微结构研究[J].植物学报,1994,36(6):418-422.
    [5]
    杨涛,陈德海,吴荔萍.安祖花组织培养及其细胞和叶绿体发育过程的电镜观察[J].亚热带植物通讯,1998,27(1):1-7.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2429) PDF downloads(437) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return