Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography

SU De-sen; CHEN Han-zhen; LIN Qiu; CHEN Hong-shan

Volume 20 Issue 4

Apr. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2005 > 20(4): 289-291

SU De-sen, CHEN Han-zhen, LIN Qiu, CHEN Hong-shan. Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography[J]. Fujian Journal of Agricultural Sciences, 2005, 20(4): 289-291.

Citation:

SU De-sen, CHEN Han-zhen, LIN Qiu, CHEN Hong-shan. Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography[J]. Fujian Journal of Agricultural Sciences, 2005, 20(4): 289-291.

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Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography

1.
Central Laboratory, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2005-05-04
Rev Recd Date: 2005-11-07
Publish Date: 2005-12-15

Abstract

Abstract

A method for the simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography was developed.The quantitative analysis on the vitamin content in fish oil was made by characterizing the retention time and spectral graph, and using the maximum absorbance wavelength for peak area.The results showed that the average recovery was 89.6%—95.0%,and variety was 3.6%—5.1%.So this method was suitable to be used in the determination on vitamin A, D and E.
- High performance liquid chromatography,
- Vitamin,
- Fish oil

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References(3)

References

[1]	Sprecher H,James A T.Biosynthesis of long chain fatty acids in mammalian systems[A].Emken EA,Dutton HJ,edi tor.Geometrical and Positional Fatty Acid Isomers[M].Champaign,IL:The American Oil Chemists' Society,1979.303-338.
[2]	骆华星,周德权.鱼油抗氧化剂的筛选[J].预防医学文献信息,2000,6(1):21-22.
[3]	GB/T 5009.82-2003,食品中维生素A和维生素E的测定[S].