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WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.
Citation:
WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.
WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.
Citation:
WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.
A pair of primers based on NS5 gene sequences of different strains of genus Flavivirus was designed for amplification of 470 bp fragments.No positive target fragment was available from H9 subtype influenza virus,Muscovy duck reovirus,duck plague,duck hepatitis virus(DHV-Ⅰ) or duck-origin avian paramyxovirus typeⅠ.The detection limit reached 20 pg under the optimized conditions.The results showed that the RT-PCR was sensitive,specific and applicable for rapid laboratory diagnosis and epidemiologic surveillance.
JACKSON G.MCNICHLOS R,FOX G,et al.Toward universal Flavivirus identification by mass cataloging[J].Journal of Molecular Diagnostics,2008,10 (2):135-141.