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Volume 21 Issue 1
Apr.  2012
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ZHOU Lun-jiang, ZHANG De-ming, LIU Yu-tao, WEI Hong, WANG Long-bai, ZHUANG Xiang-sheng. Preparation and biological characteristics of neutralizing monoclonal antibodies against newcastle disease virus[J]. Fujian Journal of Agricultural Sciences, 2006, 21(1): 39-41.
Citation: ZHOU Lun-jiang, ZHANG De-ming, LIU Yu-tao, WEI Hong, WANG Long-bai, ZHUANG Xiang-sheng. Preparation and biological characteristics of neutralizing monoclonal antibodies against newcastle disease virus[J]. Fujian Journal of Agricultural Sciences, 2006, 21(1): 39-41.

Preparation and biological characteristics of neutralizing monoclonal antibodies against newcastle disease virus

  • Received Date: 2005-08-05
  • Publish Date: 2006-03-15
  • Twenty-four monoclonal antibodies (McAbs) against newcastle disease virus (NDV) were obtained by using hybridoma technique.Among them, McAb-14, McAb-18 and McAb-21 had strong neutralization characteristics, and there was strong correspondence between the neutralization efficacity and the haemagglutination inhibition value.The McAb-18 and McAb-21 belonged to IgG2aclass, and McAb-14 was TgG2bclass.No crossreaction was detected between the McAbs and infectious bronchitis virus, Marek’s disease virus, infectious bursal disease virus, egg drop syndrome virus and infectious laryngotracheitis virus.Two of the three neutralizing McAbs were equivalent mixed randomly; the passive immunization treatment and immune preventive test of the mixture were examined in chicken.The results showed that McAb-14+McAb-18 combination was the most effective.
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    甘孟侯.中国禽病学[M].北京:中国农业出版社,1999.55-66.
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