Metabolomics analysis of mulberry fruits at different developmental stages

ZHONG Qiuzhen; LIN Qihua; ZHANG Zehuang

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ZHONG Q Z, LIN Q H, ZHANG Z H. Metabolomics analysis of mulberry fruits at different developmental stages [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−8

Citation:

ZHONG Q Z, LIN Q H, ZHANG Z H. Metabolomics analysis of mulberry fruits at different developmental stages [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−8

ZHONG Q Z, LIN Q H, ZHANG Z H. Metabolomics analysis of mulberry fruits at different developmental stages [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−8

Citation:

ZHONG Q Z, LIN Q H, ZHANG Z H. Metabolomics analysis of mulberry fruits at different developmental stages [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−8

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Metabolomics analysis of mulberry fruits at different developmental stages

Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou,　350013, China

Received Date: 2027-07-16
Rev Recd Date: 2024-09-05

Available Online: 2024-11-11

Abstract

Abstract

Objectives To study the changes in metabolic components at different development stages of mulberry fruits. Methods a widely targeted metabolomics analysis was conducted on samples from the green fruit stage, color transformation stage, and mature stage of Morus alba cv. yueshen dashi. And the KEGG metabolic pathway enrichment was then analysed based on differentially accumulated metabolites (DAMs). Results A total of 1146 metabolic components were identified from three developmental stages of mulberry fruit. Using P＜0.05 and VIP＞1.0 as the criteria, 483 metabolites were screened as DAMs, including 51 DAMs with significant differences in accumulation levels throughout the whole comparison groups. Two flavonoids and one anthocyanin were significantly increased during the development of mulberries. DAMs based KEGG metabolic pathway analysis found that α-linolenic acid metabolism and linoleic acid metabolism were significantly enriched during the green fruit development to color transition period, while ascorbate and aldarate metabolism were significantly enriched during the color transition period to maturity period, while linoleic acid metabolism and cutin, suberine and wax biosynthesis were significantly enriched throughout the fruit development process. Further analysis revealed that although there were six upaccumulated and one downaccumulated linoleic acid components in the development process of mulberry fruit, the overall accumulation level of linoleic acid showed a downward trend. Conclusions This study found that 51 DAMs might involve in the entire development process of mulberry fruits, the anthocyanins and flavonoids were significant increased, and 7 key metabolic components in the linoleic acid metabolism pathway may affect the formation of its quality and flavor. These results contributed to a better understanding of the dynamic changes in nutritional components during the ripening process of mulberries, and laid the foundation for revealing the mechanism of mulberry fruit quality and flavor formation and screening high-quality mulberry germplasm. Additionally, this research provided scientific basis for determining the picking period of mulberries.
- Mulberry,
- Fruit development,
- Metabolomics,
- KEGG metabolic pathway,
- Linoleic acid

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References(22)

References

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