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LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song. Optimization of ISSR-PCR reaction system in tea plant[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 247-252.
Citation:
LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song. Optimization of ISSR-PCR reaction system in tea plant[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 247-252.
LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song. Optimization of ISSR-PCR reaction system in tea plant[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 247-252.
Citation:
LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song. Optimization of ISSR-PCR reaction system in tea plant[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 247-252.
The optimal ISSR-PCR reaction conditions in tea was established by studying the main parameters.Results showed that the optimal concentration of DNA,Taq polymerase,primer,Mg2+,dNTp in 20 μl reaction mixture was 40 ng,0.5 U,0.25 μmol·L-1,1.5 mmol·L-1,0.2 mmol·L-1.The appropriate annealing temperature was 2-3℃ higher than Tm of corresponding primer.