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Volume 21 Issue 3
Apr.  2012
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Article Contents
CHE Yong-liang, CHEN Shao-ying, WEI Hong, WANG Long-bai, CHEN Shi-long, ZHOU Lun-jiang, ZHUANG Xiang-sheng. Development of RT-PCR method for detecting swine janpanese encephalitis virus[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 228-230.
Citation: CHE Yong-liang, CHEN Shao-ying, WEI Hong, WANG Long-bai, CHEN Shi-long, ZHOU Lun-jiang, ZHUANG Xiang-sheng. Development of RT-PCR method for detecting swine janpanese encephalitis virus[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 228-230.

Development of RT-PCR method for detecting swine janpanese encephalitis virus

  • Received Date: 2006-05-29
  • Rev Recd Date: 2006-08-29
  • Publish Date: 2006-09-15
  • A pair of primers were designed and synthesized based on the sequences of the JEV genome,and the RT-PCR method to detect the Japanese Encephalitis Virus(JEV) were development.The 430 bp specifical fragment were obtained from the JEV vaccine strains RNA by this RT-PCR.The test of the gene sequence of RT-PCR product achieved to 98%-100% with that of other JEV strains.The sensitivity of RT-PCR reached to 10 pg JEV RNA.These results showed that the RT-PCR method to detect JEV were of better specificity and higher sensibility.
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    吴光华,杨佩英,唐家琪.八种重要传染病的防治[M].第1版.北京:人民军医出版社,2001:129-130.
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    陈伯权,刘琴兰,孙月英,等.单克隆抗体反向被动血凝和反向被动血凝抑制试验在乙脑快速诊断中的应用[J].中华微生物和免疫学杂志,1987,7(1):43-45.
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