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HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Citation:
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Citation:
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Different extraction methods were compared in extracting of active substances from metabolites of Brevibacillus brevis FJAT-0809-GLX.It macroporous tesin method provided the best effect in extraction with 1.593 gL-1.Antibacterial activities of the extract was higher in it by macroporous resin method than it by the chloroform.The optimal procedures applied for extraction of active substances were: centrifuging the fermentation broth of FJAT-0809-GLX at 3600 rpm for 30 min,mixing the supernatant with 40 gL-1 macroporous resin(AmberliteXAD16)and then vortexing for 4h at 160 rpm,28℃;filling the column with the mixture and eluting it with acetone in the ratio of 3 times in given volum of extraction.The eluent was collected and concentrated rotary evaporation at 40℃.