• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 26 Issue 4
Nov.  2011
Turn off MathJax
Article Contents
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Citation: HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.

Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX

  • Received Date: 2011-01-02
  • Rev Recd Date: 2011-06-19
  • Publish Date: 2011-08-15
  • Different extraction methods were compared in extracting of active substances from metabolites of Brevibacillus brevis FJAT-0809-GLX.It macroporous tesin method provided the best effect in extraction with 1.593 gL-1.Antibacterial activities of the extract was higher in it by macroporous resin method than it by the chloroform.The optimal procedures applied for extraction of active substances were: centrifuging the fermentation broth of FJAT-0809-GLX at 3600 rpm for 30 min,mixing the supernatant with 40 gL-1 macroporous resin(AmberliteXAD16)and then vortexing for 4h at 160 rpm,28℃;filling the column with the mixture and eluting it with acetone in the ratio of 3 times in given volum of extraction.The eluent was collected and concentrated rotary evaporation at 40℃.
  • loading
  • [1]
    李一青,刘树芳,李铭刚,等. 藤黄灰链霉菌 ECO00001 菌株中寡霉素 A 和 C 的分离鉴定及其活性[J]. 植物保护学报, 2008, 35(1):47-50.
    [2]
    [3]
    蔡寅,张芳,顾觉奋. 大孔吸附树脂在微生物制药分离纯化应用上的最新进展[J]. 离子交换与吸附, 2008, 24(5):473-480.
    [4]
    [5]
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2597) PDF downloads(749) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return