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Volume 26 Issue 4
Nov.  2011
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Article Contents
YANG Shao-hua, LIU Hua-qing, WANG Feng. Genetic Analysis and Gene Mapping of a Spotted Leaf Mutant W1764 in Rice[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 519-522.
Citation: YANG Shao-hua, LIU Hua-qing, WANG Feng. Genetic Analysis and Gene Mapping of a Spotted Leaf Mutant W1764 in Rice[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 519-522.

Genetic Analysis and Gene Mapping of a Spotted Leaf Mutant W1764 in Rice

  • Received Date: 2011-03-21
  • Rev Recd Date: 2011-05-11
  • Publish Date: 2011-08-15
  • A non-T-DNA insertion spotted leaf mutant W1764 was identified in a transgenic offspring of rice(Oryza sativa L.)indica cv.Minghui86.The spot phenotype was first appearing at the tip part of the lowest leaf at late stage of tillering.As the plant growing,the spot spread from tip part to the whole leaves from lower leaves to the upper ones.When the mutant plant matured,the spot spread to the whole plant.Genetic analysis showed that the mutant phenotype was controlled by a single recessive gene.F2 population build up with mutant W1764 and hybrid rice varieties 02428,and the 136 mutant phenotypes were used for gene targeting,we mapped the mutant gene in 1.9 cM interval between DNA marker NSSR1229 and L05-78 on the long arm of chromosome 12.We temporarily named the gene as spotted leaf 30(t)[spl30(t)].
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    YAMANOUCHI U, YANO M, LIN H, et al.A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein[J].Proc Natl Acad Sci,2002, 99(11):7530-7535.
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    MORI M, TOMITA C, SUGIMOTO K, et al.Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice[J].Plant Mol Biol,2007, 63(6):847-860.
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    QIAO Y, JIANG W, LEE J, et al.SPL28encodes a clathrin-associated adaptor protein complex 1, medium subunit 1(A P1M1)and is responsible for spotted leaf and early senescence in rice(Oryza sativa)[J].The New phytologist, 2010, 185(1):258-274.
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    MICHELMORE RW, PARAN I, KESSELI RV.Identification of markers linked to disease-resistance genes by bulked segregant analysis : A rapid method to detect markers in specific genomic regions by using segregating populations[J]. Proc Natl Acad Sci,1991, 88(21):9828-9832.
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