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Volume 21 Issue 4
Apr.  2012
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Article Contents
GONG hui, LIN Tian-long, WU Zong-fu, SONG Tie-ying. Immunity level of the eel after oral immunized with β-hemA-ISCOMs[J]. Fujian Journal of Agricultural Sciences, 2006, 21(4): 325-329.
Citation: GONG hui, LIN Tian-long, WU Zong-fu, SONG Tie-ying. Immunity level of the eel after oral immunized with β-hemA-ISCOMs[J]. Fujian Journal of Agricultural Sciences, 2006, 21(4): 325-329.

Immunity level of the eel after oral immunized with β-hemA-ISCOMs

  • Received Date: 2006-04-09
  • Rev Recd Date: 2006-11-16
  • Publish Date: 2006-12-15
  • The recombinant plasmid containing completed β-hemolysin gene of Aeromonas hydrophila was transformed into E.coli DH5α and could express β-hemolysin with native biological function.The ISCOMs included crude purified transformant’s extracellular products (ECPs) was made and named β-hemA-ISCOMs.The result showed it could enhanced the proliferation ability of eel’s lymphocyte and induced immunity significantly by oral administration (P0.05),after challenge,the livability of first immunized was 44.4%.the livability of second immunized was 100%.This was, to our knowledge,the first report about ISCOMs used in eel’s oral administration,indicated that ISCOMs technology could be widely used for producing fish oral vaccines.
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    OAEL HOGLAND,GELDERBLOM.Quaternary structure of the immunstimulating complex iscom[J].Ultrastruct Mol Struct Res,1989,102:240-248.
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